Western blot analysis of EBI3 in 1) Daudi cell lysate using 2.0 ug/ml, 2) Ramos cell lysate at 2.0 ug/ml in the absence of immunizing peptide 3) Ramos cell lysate at 2.0 ug/ml in the presence of immunizing peptide and 4) RAW cell lysate at 5.0 ug/ml using a EBI3 polyclonal antibody (Product # PA5-23228)
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (73 RGHSWPCLQQTPTSTSC 89) of EBI3 protein.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||2-6 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
EBI3 is a novel hematopoietin receptor family member related to the p40 subunit of interleukin-12 and to the ciliary neurotrophic factor receptor. It's expression is induced in B lymphocytes by Epstein-Barr virus (EBV) infection. Human EBI3 gene maps to chromosome 19p13.3, near genes encoding the erythropoietin receptor and the cytokine receptor-associated kinase, Tyk2. The protein is expressed in vivo by scattered cells in interfollicular zones of tonsil tissue, by cells associated with sinusoids in perifollicular areas of spleen tissue, and at very high levels by placental syncytiotrophoblasts. EBI3 expression in vitro is induced in EBV-negative cell lines by expression of the EBV latent infection membrane protein-1 and in peripheral blood mononuclear cells by pokeweed mitogen stimulation. The protein plays a crucial role in regulating cell-mediated immune responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.