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Invitrogen
EBP50 Polyclonal Antibody
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FIGURE: 1 / 37
EBP50 Antibody (PA1-090) in ICC/IF
Immunofluorescent analysis of EBP50 was performed using 70% confluent log phase T-47D cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with EBP50 Rabbit Polyclonal Antibody (Product # PA1-090) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2... View More
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.jpg?time=20220826?time=20220826 "EBP50 Antibody (PA1-090) in IHC (P)")
Product Details
PA1-090
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Dog,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Bacterially expressed, His-tagged human EBP50.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-090 detects ezrin-radixin-moesin (ERM) binding phosphoprotein of 50 kDa (EBP50) from mouse tissues, human T84 and Calu3 cells as well as recombinant human protein.
PA1-090 has been successfully used in Western blot, Immunohistochemistry, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~50 kDa protein representing EBP50 from T84 cell extract. Immunohistochemical staining of EBP50 in mouse airway results in staining of the epithelium.
The PA1-090 immunogen is recombinant human His-tagged EBP50 obtained from bacteria. All His-tag specific antibody has been removed. This product was epitope affinity purified using a synthetic peptide to residues C R(286) S A S S D T S E E L N S Q D S P(302) of human EBP50.
Target Information
NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: EBP50; ezrin-radixin-moesin binding phosphoprotein-50; Ezrin-radixin-moesin-binding phosphoprotein 50; Na(+)/H(+) exchange regulatory cofactor 1; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na+/H+ exchange regulatory co-factor; NHE-RF; NHERF-1; Regulatory cofactor of Na(+)/H(+) exchanger; SLC9A3 regulator 1; So; Sodium-hydrogen exchanger regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1; Solute carrier family 9 isoform A3 regulatory factor 1; solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1; unnamed protein product
Gene Aliases: EBP-50; EBP50; NHE-RF; NHERF; NHERF-1; NHERF1; NPHLOP2; SLC9A3R1
UniProt ID: (Human) O14745, (Mouse) P70441
Entrez Gene ID: (Human) 9368, (Mouse) 26941
receptor binding
protein binding
beta-catenin binding
gamma-aminobutyric acid transmembrane transporter activity
chloride channel regulator activity
phosphatase binding
PDZ domain binding
beta-2 adrenergic receptor binding
type 2 metabotropic glutamate receptor binding
type 3 metabotropic glutamate receptor binding
identical protein binding
protein anchor
dopamine receptor binding
binding, bridging
growth factor receptor binding
channel activator activity
protein domain specific binding
protein complex binding
protein complex scaffold
protein self-association
morphogenesis of an epithelium
renal sodium ion transport
sodium ion transport
plasma membrane organization
nuclear migration
adenylate cyclase-activating dopamine receptor signaling pathway
sensory perception of sound
protein localization
negative regulation of cell proliferation
regulation of cell shape
regulation of cell size
negative regulation of platelet-derived growth factor receptor signaling pathway
fibroblast migration
negative regulation of fibroblast migration
negative regulation of sodium ion transport
Wnt signaling pathway
gland morphogenesis
microvillus assembly
actin cytoskeleton organization
cellular phosphate ion homeostasis
bile acid secretion
glutathione transport
cilium organization
establishment of epithelial cell apical/basal polarity
maintenance of epithelial cell apical/basal polarity
regulation of protein kinase activity
negative regulation of mitotic cell cycle
establishment of localization in cell
establishment of Golgi localization
negative regulation of protein kinase B signaling
gamma-aminobutyric acid import
auditory receptor cell stereocilium organization
phospholipase C-activating dopamine receptor signaling pathway
protein-containing complex assembly
renal absorption
negative regulation of ERK1 and ERK2 cascade
protein localization to plasma membrane
negative regulation of canonical Wnt signaling pathway
cerebrospinal fluid circulation
renal phosphate ion absorption
import across plasma membrane
transport across blood-brain barrier
regulation of renal phosphate excretion
negative regulation of cell motility
positive regulation of intrinsic apoptotic signaling pathway
negative regulation of phosphatidylinositol 3-kinase signaling
cAMP-mediated signaling
negative regulation of cell migration
negative regulation of sodium:proton antiporter activity
cellular protein localization
regulation of excretion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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