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Invitrogen
EBP50 Polyclonal Antibody
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-17045
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide surrounding Ala310 of human NHERF1
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
10 µg/mL
Amount
1 µg
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: EBP50; ezrin-radixin-moesin binding phosphoprotein-50; Na(+)/H(+) exchange regulatory cofactor 1; Na+/H+ exchange regulatory co-factor; NHE-RF; regulatory cofactor of Na(+)/H(+) exchanger; SLC9A3 regulator 1; So; solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1; unnamed protein product
Gene Aliases: EBP50; NHE-RF; NHERF; NHERF-1; NPHLOP2; SLC9A3R1
UniProt ID: (Human) O14745
Entrez Gene ID: (Human) 9368
receptor binding
protein binding
beta-catenin binding
gamma-aminobutyric acid transmembrane transporter activity
chloride channel regulator activity
phosphatase binding
PDZ domain binding
beta-2 adrenergic receptor binding
type 2 metabotropic glutamate receptor binding
type 3 metabotropic glutamate receptor binding
identical protein binding
protein anchor
dopamine receptor binding
binding, bridging
growth factor receptor binding
channel activator activity
morphogenesis of an epithelium
plasma membrane organization
nuclear migration
negative regulation of cell proliferation
regulation of cell shape
regulation of cell size
negative regulation of platelet-derived growth factor receptor signaling pathway
gland morphogenesis
microvillus assembly
bile acid secretion
glutathione transport
cilium organization
establishment of epithelial cell apical/basal polarity
maintenance of epithelial cell apical/basal polarity
regulation of protein kinase activity
negative regulation of mitotic cell cycle
establishment of Golgi localization
negative regulation of protein kinase B signaling
gamma-aminobutyric acid import
protein-containing complex assembly
renal absorption
negative regulation of ERK1 and ERK2 cascade
protein localization to plasma membrane
negative regulation of canonical Wnt signaling pathway
cerebrospinal fluid circulation
renal phosphate ion absorption
import across plasma membrane
transport across blood-brain barrier
negative regulation of cell motility
positive regulation of intrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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