Invitrogen

EBP50 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

2 Published Figures

1 Reference

View all (12) EBP50 antibodies

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Datasheet

Protocols

Questions & Answers

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EBP50 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of NHERF1 in MCF-7 cells using a NHERF1 polyclonal antibody (Product # PA5-17045) (green) showing staining at the apical membrane. DNA is labeled using a fluorescent blue dye.

EBP50 Antibody in Western Blot (WB) — Western blot was performed using Anti-EBP50 Monoclonal Antibody (Product # PA5-17045) and a 50 kDa band corresponding to EBP50 was observed across cell lines tested. Membrane enriched extracts (30 µg lysate) of MCF-7(Lane 1), T-47D ( Lane 2), SK-BR-3 (Lane 3), MDA-MB-231 (Lane 4), HeLa (Lane 5), Hep G2 (Lane 6), and PC-12 ( Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 Dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

EBP50 Antibody in Immunohistochemistry (IHC) — Figure 1 Immunohistochemistry showing EBP50 staining in GC. Sample of (A) negative EBP50 protein expression, (B) a positive case scoring 1+; (C) a positive case scoring 2+, and (D) a positive case scoring 3+ [magnification, x400; objective lens; Streptomyces avidin-peroxidase connection (SP) method]. EBP50, ezrin-radixin-moesin-binding phosphoprotein 50; GC, gastric cancer.

EBP50 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of EBP50 was observed in MCF-7, SK-BR-3 and T-47D in higher levels in comparison to MDA-MB-231 in low levels using Anti-EBP50 Monoclonal Antibody (Product # PA5-17045) in Western Blot. (doi:https://doi.org/10.3892/or.2017.5649). {RE}

Product Details

PA5-17045

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	-
Immunocytochemistry (ICC/IF)	1:50	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide surrounding Ala310 of human NHERF1 View immunogen
Conjugate	Unconjugated
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains	no preservative
Storage conditions	-20°C
RRID	AB_11009365

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Clinical significance of EBP50 overexpression assessed by quantum dot analysis in gastric cancer.

Oncology letters

Lv XG,Lei XF,Ji MY,Guo XF,Wang J,Dong WG

PA5-17045 was used in immunohistochemistry to study the prognostic value of EBP50 overexpression in gastric cancer

Sat Jun 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: EBP50; ezrin-radixin-moesin binding phosphoprotein-50; Ezrin-radixin-moesin-binding phosphoprotein 50; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na+/H+ exchange regulatory co-factor; NHERF-1; Regulatory cofactor of Na(+)/H(+) exchanger; So; Sodium-hydrogen exchanger regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1; Solute carrier family 9 isoform A3 regulatory factor 1; solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1

Gene Aliases: EBP50; NHERF; NHERF-1; NHERF1; NPHLOP2; SLC9A3R1

UniProt ID: (Human) O14745

Entrez Gene ID: (Human) 9368

Function(s)

Process(es)

receptor binding protein binding beta-catenin binding chloride channel regulator activity phosphatase binding PDZ domain binding beta-2 adrenergic receptor binding protein complex binding protein complex scaffold protein self-association dopamine receptor binding growth factor receptor binding

EBP50 Polyclonal Antibody

Product Details

PA5-17045

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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