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Invitrogen
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:200-1:1,000 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Peptide (C)IQKENSREALVEPA, corresponding to residues 261-274 of rat NHERF-1 |
Conjugate |
Unconjugated |
Form |
Lyophilized |
Concentration |
0.2 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.4, with 1% BSA, 5% sucrose |
Contains |
0.025% sodium azide |
Storage conditions |
-20°C |
RRID |
AB_2735750 |
Product is shipped at room temperature as a lyophilized powder and should be stored at -20 °C upon receipt. Reconstitution: add 50 µL of deionized water.
NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: EBP50; ERM-binding phosphoprotein; Ezrin-radixin-moesin-binding phosphoprotein 50; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; NHERF-1; Regulatory cofactor of Na(+)/H(+) exchanger; So; Sodium-hydrogen exchanger regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1; solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1; Solute carrier family 9 isoform A3 regulatory factor 1
Gene Aliases: Nherf; Nherf1; Slc9a3r1
UniProt ID: (Rat) Q9JJ19
Entrez Gene ID: (Rat) 59114
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