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ECE1 Antibody (PA5-102821) in ICC/IF
Immunocytochemistry analysis of ECE1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ECE1 polyclonal antibody (Product # PA5-102821) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-102821
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ECE1(Accession P42892), corresponding to amino acid residues T118-T168.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2852211
Product Specific Information
Antibody detects endogenous levels of total ECE1.
Target Information
Endothelin-converting enzyme 1 (ECE1) is located on chromosome 1p36.12 and encodes a zinc metalloprotease involved in the maturation of endothelin peptides, which are critical for vascular tone regulation and blood pressure control. ECE1 processes precursor proteins into active endothelins, particularly endothelin-1, the most potent vasoconstrictor known. This enzyme is expressed in multiple tissues, including endothelial cells, smooth muscle cells, and the central nervous system, where it participates in diverse physiological processes such as vasoconstriction, cell proliferation, and hormone secretion. Beyond its cardiovascular functions, ECE1 is implicated in neural development and neural crest cell migration. Dysregulation of ECE1 activity or endothelin signaling is associated with several pathologies, including hypertension, heart failure, and cancer progression due to its role in controlling vascular dynamics. Research into ECE1 aims to explore its therapeutic potential in managing cardiovascular disorders and its broader implications in systemic pathophysiology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ECE 1; ECE-1; Endothelin-converting enzyme 1; endothelin-converting enzyme-1a; endothelin-converting enzyme-1b; endothelin-converting enzyme-1d
Gene Aliases: AW322500; ECE; ECE-1; ECE-1a; ECE-1b; ECE-1d; ECE1
UniProt ID: (Human) P42892, (Mouse) Q4PZA2
Entrez Gene ID: (Human) 1889, (Rat) 94204, (Mouse) 230857
response to hypoxia
positive regulation of receptor recycling
regulation of systemic arterial blood pressure by endothelin
apoptotic process
heart development
positive regulation of cardiac muscle hypertrophy
substance P catabolic process
bradykinin catabolic process
calcitonin catabolic process
protein processing
peptide hormone processing
regulation of vasoconstriction
positive regulation of cAMP biosynthetic process
endothelin maturation
response to muscle stretch
regulation of vasodilation
hormone catabolic process
response to drug
embryonic digit morphogenesis
ear development
positive regulation of G-protein coupled receptor protein signaling pathway
response to cadmium ion
pharyngeal system development
regulation of ERK1 and ERK2 cascade
proteolysis
regulation of blood pressure
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