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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:1,000-1:2,000 | - |
Immunocytochemistry (ICC/IF) |
1:50 - 1:200 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 28-290 of human ECHS1. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
3.55 mg/mL |
Purification |
Affinity Chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2808061 |
Immunogen sequence: ASGANFEYII AEKRGKNNTV GLIQLNRPKA LNALCDGLID ELNQALKTFE EDPAVGAIVL TGGDKAFAAG ADIKEMQNLS FQDCYSSKFL KHWDHLTQVK KPVIAAVNGY AFGGGCELAM MCDIIYAGEK AQFAQPEILI GTIPGAGGTQ RLTRAVGKSL AMEMVLTGDR ISAQDAKQAG LVSKICPVET LVEEAIQCAE KIASNSKIVV AMAKESVNAA FEMTLTEGSK LEKKLFYSTF ATDDRKEGMT AFVEKRKANF KDQ; Positive Samples: LO2, 293T, U-87MG, MCF7, 22Rv1, Mouse liver, Mouse kidney, Mouse heart, Rat liver; Cellular Location: Mitochondrion matrix
The protein encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ECHS1; enoyl CoA hydratase, short chain, 1, mitochondrial; enoyl Coenzyme A hydratase, short chain 1; enoyl Coenzyme A hydratase, short chain, 1, mitochondrial; Enoyl-CoA hydratase 1; Enoyl-CoA hydratase short chain 1 mitochondrial; Enoyl-CoA hydratase, mitochondrial; Enoyl-CoA hydratase, short chain 1, mitochondrial; enoyl-CoA hydratase, short chain, 1, mitochondrial; mECH; mitochondrial short-chain enoyl-coenzyme A hydratase 1; SCEH; short chain enoyl-CoA hydratase; Short-chain enoyl-CoA hydratase
Gene Aliases: C80529; ECHS1; ECHS1D; SCEH
UniProt ID: (Human) P30084, (Rat) P14604, (Mouse) Q99LX7
Entrez Gene ID: (Human) 1892, (Rat) 140547, (Mouse) 93747
Molecular Function:
hydratase
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