Proteintech

ECM1 Polyclonal Antibody

View all (18) ECM1 antibodies

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ECM1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 11521-1-AP (ECM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ECM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human thyroid cancer tissue slide using 11521-1-AP ( ECM1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

ECM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human thyroid cancer tissue slide using 11521-1-AP ( ECM1 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

ECM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 10x lens).

ECM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 40x lens).

ECM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human thyroid cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 40x lens).

ECM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human thyroid cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 10x lens).

ECM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 25x lens).

ECM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 40x lens).

ECM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 10x lens).

ECM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human thyroid cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 40x lens).

ECM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human thyroid cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 10x lens).

ECM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 40x lens).

ECM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 10x lens).

ECM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 25x lens).

ECM1 Antibody in Western Blot (WB) — Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ECM1 Antibody in Western Blot (WB) — Rat pancreas tissue were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ECM1 Antibody in Western Blot (WB) — Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ECM1 Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ECM1 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ECM1 Antibody in Western Blot (WB) — A549 cells were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

ECM1 Antibody in Western Blot (WB) — MCF7 cells were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ECM1 Antibody in Western Blot (WB) — L02 cells were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Product Details

11521-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 4 publications 4 publications
Immunohistochemistry (IHC)	-	View 7 publications 7 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:400	-
Immunocytochemistry (ICC/IF)	1:50-1:500	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Dog, Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ECM1 Fusion Protein Ag2084 (241-540 aa encoded by BC023505) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.19 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MSRFCEAEF SVKTRPHWCC TRQGEARFSC FQEEAPQPHY QLRACPSHQP DISSGLELPF PPGVPTLDNI KNICHLRRFR SVPRNLPATD PLQRELLALI QLEREFQRCC RQGNNHTCTW KAWEDTLDKY CDREYAVKTH HHLCCRHPPS PTRDECFARR APYPNYDRDI LTIDISRVTP NLMGHLCGNQ RVLTKHKHIP GLIHNMTARC CDLPFPEQAC CAEEEKLTFI NDLCGPRRNI WRDPALCCYL SPGDEQVNCF NINYLRNVAL VSGDTENAKG QGEQGSTGGT NISSTSEPKE E (241-540 aa encoded by BC023505)

Target Information

Secretory Component is an epithelial transport receptor for polymeric IgA and IgM. Antibody to secretory component has been shown to be useful for identifying glandular carcinomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Uterine and placental distribution of selected extracellular matrix (ECM) components in the dog.

Reproduction (Cambridge, England)

Graubner FR,Boos A,Aslan S,Kücükaslan I,Kowalewski MP

Tue May 01 00:00:00 EDT 2018

Extracellular matrix protein 1 promotes cell metastasis and glucose metabolism by inducing integrin β4/FAK/SOX2/HIF-1α signaling pathway in gastric cancer.

Oncogene

Gan L,Meng J,Xu M,Liu M,Qi Y,Tan C,Wang Y,Zhang P,Weng W,Sheng W,Huang M,Wang Z

Thu Feb 08 00:00:00 EST 2018

Spatial Control of Primary Ciliogenesis by Subdistal Appendages Alters Sensation-Associated Properties of Cilia.

Developmental cell

Mazo G,Soplop N,Wang WJ,Uryu K,Tsou MF

Mon Nov 21 00:00:00 EST 2016

ECM1 promotes migration and invasion of hepatocellular carcinoma by inducing epithelial-mesenchymal transition.

World journal of surgical oncology

Chen H,Jia W,Li J

Wed Jul 27 00:00:00 EDT 2016

ECM1 promotes migration and invasion of hepatocellular carcinoma by inducing epithelial-mesenchymal transition.

World journal of surgical oncology

Chen H,Jia W,Li J

Wed Jul 27 00:00:00 EDT 2016

Differential expression of connective tissue growth factor and extracellular matrix proteins in lichen sclerosus.

Journal of the European Academy of Dermatology and Venereology : JEADV

Gambichler T,Skrygan M,Czempiel V,Tigges C,Kobus S,Meier JJ,Köhler CU,Scola N,Stücker M,Altmeyer P,Kreuter A

Wed Feb 01 00:00:00 EST 2012

Extracellular matrix protein 1, a novel prognostic factor, is associated with metastatic potential of hepatocellular carcinoma.

Medical oncology (Northwood, London, England)

Chen H,Jia WD,Li JS,Wang W,Xu GL,Ma JL,Ren WH,Ge YS,Yu JH,Liu WB,Zhang CH,Wang YC

Thu Dec 01 00:00:00 EST 2011

Effects of dehydroepiandrosterone on proliferation, migration, and death of breast cancer cells.

European journal of pharmacology

López-Marure R,Contreras PG,Dillon JS

Sat Jun 25 00:00:00 EDT 2011

Expression of extracellular matrix proteins in reticular variant of mid-dermal elastolysis.

Journal of the European Academy of Dermatology and Venereology : JEADV

Gambichler T,Stücker M,Kreuter A,Matip R,Gaifullina R,Scola N,Skrygan M

Wed Dec 01 00:00:00 EST 2010

Phospholipid scramblase 1 is secreted by a lipid raft-dependent pathway and interacts with the extracellular matrix protein 1 in the dermal epidermal junction zone of human skin.

The Journal of biological chemistry

Merregaert J,Van Langen J,Hansen U,Ponsaerts P,El Ghalbzouri A,Steenackers E,Van Ostade X,Sercu S

Fri Nov 26 00:00:00 EST 2010

Phospholipid scramblase 1 is secreted by a lipid raft-dependent pathway and interacts with the extracellular matrix protein 1 in the dermal epidermal junction zone of human skin.

The Journal of biological chemistry

Merregaert J,Van Langen J,Hansen U,Ponsaerts P,El Ghalbzouri A,Steenackers E,Van Ostade X,Sercu S

Fri Nov 26 00:00:00 EST 2010

Quantitative proteomic analysis by iTRAQ(R) for the identification of candidate biomarkers in ovarian cancer serum.

Proteome science

Boylan KL,Andersen JD,Anderson LB,Higgins L,Skubitz AP

Mon Jun 14 00:00:00 EDT 2010

ECM1 interacts with fibulin-3 and the beta 3 chain of laminin 332 through its serum albumin subdomain-like 2 domain.

Matrix biology : journal of the International Society for Matrix Biology

Sercu S,Lambeir AM,Steenackers E,El Ghalbzouri A,Geentjens K,Sasaki T,Oyama N,Merregaert J

Wed Apr 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: Extracellular matrix protein 1; RP11-54A4.6; Secretory component p85; testicular tissue protein Li 61

Gene Aliases: AI663821; ECM1; p85; URBWD

UniProt ID: (Human) Q16610, (Mouse) Q3U5Q5, (Rat) Q62894

Entrez Gene ID: (Human) 1893, (Mouse) 13601, (Rat) 116662

ossification angiogenesis positive regulation of endothelial cell proliferation negative regulation of cytokine-mediated signaling pathway platelet degranulation regulation of type 2 immune response regulation of transcription from RNA polymerase II promoter inflammatory response signal transduction negative regulation of peptidase activity negative regulation of bone mineralization biomineral tissue development positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of angiogenesis regulation of T cell migration regulation of bone mineralization biological_process

Process(es)

protease binding signal transducer activity interleukin-2 receptor binding protein binding protein C-terminus binding enzyme binding laminin binding molecular_function