Proteintech

ECM1 Polyclonal Antibody

View all (18) ECM1 antibodies

Questions & Answers

Questions & Answers

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ECM1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 11521-1-AP (ECM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ECM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human thyroid cancer tissue slide using 11521-1-AP ( ECM1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

ECM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human thyroid cancer tissue slide using 11521-1-AP ( ECM1 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

ECM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 10x lens).

ECM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 40x lens).

ECM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human thyroid cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 40x lens).

ECM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human thyroid cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 10x lens).

ECM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 25x lens).

ECM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 40x lens).

ECM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 10x lens).

ECM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human thyroid cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 40x lens).

ECM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human thyroid cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 10x lens).

ECM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 40x lens).

ECM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 10x lens).

ECM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 25x lens).

ECM1 Antibody in Western Blot (WB) — Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ECM1 Antibody in Western Blot (WB) — Rat pancreas tissue were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ECM1 Antibody in Western Blot (WB) — Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ECM1 Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ECM1 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ECM1 Antibody in Western Blot (WB) — A549 cells were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

ECM1 Antibody in Western Blot (WB) — MCF7 cells were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ECM1 Antibody in Western Blot (WB) — L02 cells were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

-

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

-

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Dog, Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ECM1 Fusion Protein Ag2084

Conjugate

Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Target Information

Secretory Component is an epithelial transport receptor for polymeric IgA and IgM. Antibody to secretory component has been shown to be useful for identifying glandular carcinomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Extracellular matrix protein 1; RP11-54A4.6; Secretory component p85; testicular tissue protein Li 61

Gene Aliases: AI663821; ECM1; p85; URBWD

UniProt ID: (Human) Q16610, (Mouse) Q61508, (Rat) Q62894

Entrez Gene ID: (Human) 1893, (Mouse) 13601, (Rat) 116662

ossification angiogenesis positive regulation of endothelial cell proliferation negative regulation of cytokine-mediated signaling pathway platelet degranulation regulation of type 2 immune response regulation of transcription from RNA polymerase II promoter inflammatory response signal transduction negative regulation of peptidase activity negative regulation of bone mineralization biomineral tissue development positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of angiogenesis regulation of T cell migration regulation of bone mineralization biological_process

Process(es)

protease binding signal transducer activity interleukin-2 receptor binding protein binding protein C-terminus binding enzyme binding laminin binding molecular_function