Invitrogen

ECT2 Polyclonal Antibody

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ECT2 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Fig. 3 Downregulation of ECT2 inhibits the proliferation and migration of HCC cells. A Representative immunohistochemistry stain of ECT2 in HCC and para-cancerous normal tissues. B Quantification of PCR analysis of ECT2 expression in HCC and para-cancerous normal tissues. C Western blot analysis of ECT2 after transfection of ECT2-targeting shRNA. D ECT2 downregulation inhibits cell proliferation. Cancer cells were transfected with ECT2-targeting shRNA, and the absorption (A450 nm) was detected at 0, 24, 48, and 72 h. E Cancer cells transfected with ECT2-targeting shRNA and the normal control were assayed for clonogenicity in adherent cultures. F EdU incorporation assay was used to examine the proliferation of cancer cells after ECT2 knockdown. G Transwell assay was used for cancer cells transfected with ECT2-targeting shRNA (magnification 100x). H Wound-healing assay was used for cancer cells transfected with ECT2-targeting shRNA (magnification 50x, scale bar: 500 mum). Error bars represent means +- SD. * p < 0.05, ** p < 0.01. NS means ""not significant"" by paired two-sided Student's t -test.

ECT2 Antibody in Western Blot (WB) — Fig. 6 ECT2 overexpression increases the production of lactic acid and subsequently promotes M2 macrophage polarization. A Correlation analysis between ECT2 and macrophages. B Western blot analysis of p-AKT, PLK1, and AKT expression levels after ECT2 was overexpressed in the HepG2 cell line. C Lactate acid production by cancer cells was evaluated after ECT2 was overexpressed. D Immunofluorescence analysis of macrophage polarization markers. E Western blot analysis was used to evaluate the effect of LDHA knockdown. F Lactate acid production by cancer cells was evaluated after ECT2 was overexpressed and/or LDHA was knocked down. G CD206 and CD163, which are polarization markers, were evaluated by flow cytometry after macrophages were co-cultured with cancer cells whose LDHA and/or ECT2 expression was influenced. Error bars represent means +- SD. * p < 0.05, ** p < 0.01. NS means ""not significant"" by paired two-sided Student's t -test.

Product Details

PA5-67612

Applications	Tested Dilution	Publications
Western Blot (WB)	-	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:100	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Not Applicable
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthesized peptide derived from human ECT2.
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	affinity chromatography
Storage buffer	PBS, pH 7.4, with 40% glycerol
Contains	0.05% sodium azide
Storage conditions	-20°C
RRID	AB_2688413

Product Specific Information

Predicted to react with Mouse samples.

Target Information

The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ECT2 overexpression promotes the polarization of tumor-associated macrophages in hepatocellular carcinoma via the ECT2/PLK1/PTEN pathway.

Cell death & disease

Xu D,Wang Y,Wu J,Zhang Z,Chen J,Xie M,Tang R,Chen C,Chen L,Lin S,Luo X,Zheng J

Published figure using ECT2 polyclonal antibody (Product # PA5-67612) in Immunohistochemistry

Mon Feb 08 00:00:00 EST 2021

ECT2 overexpression promotes the polarization of tumor-associated macrophages in hepatocellular carcinoma via the ECT2/PLK1/PTEN pathway.

Cell death & disease

Xu D,Wang Y,Wu J,Zhang Z,Chen J,Xie M,Tang R,Chen C,Chen L,Lin S,Luo X,Zheng J

Published figure using ECT2 polyclonal antibody (Product # PA5-67612) in Immunohistochemistry

Mon Feb 08 00:00:00 EST 2021

Bioinformatics

Protein Aliases: epithelial cell transforming sequence 2 oncogene; Epithelial cell-transforming sequence 2 oncogene; FLJ10461; MGC138291; Protein ECT2

Gene Aliases: AI528536; ARHGEF31; ECT2; mKIAA4037

UniProt ID: (Human) Q9H8V3, (Mouse) Q07139

Entrez Gene ID: (Human) 1894, (Mouse) 13605

Molecular Function: G-protein modulator protein-binding activity modulator guanyl-nucleotide exchange factor