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Bethyl Laboratories
EDD1 Polyclonal Antibody
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FIGURE: 1 / 1
EDD1 Antibody (A303-045A) in WB
Detection of human and mouse EDD1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (H; 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (5, 15, and 50 µg for WB) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-EDD1 antibody (Product # A303-045A) used for WB at 0.4 µg/mL (A) and 1 µg/mL (B) and used for IP at 6 µg/mg lysate. EDD1 was less efficiently immunoprecipitated by rabbit anti-EDD1 antibody A300-573A, which recognizes an upstream epitope. Detection: Chemilumine... View More
Product Details
A303-045A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A region between residue 2749 and 2799 of human E3 Ubiquitin Protein Ligase, HECT domain.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Rat
Target Information
EDD (for E3 identified by Differential Display) is a progestin-regulated gene that was isolated from T-47D human breast cancer cells. Based on sequence homology, EDD appears to be a human homolog of the Drosophila hyperplastic discs (hyd) gene, a tumor suppressor gene that is required for control of imaginal disc growth. EDD contains a HECT domain in the carboxy terminus. HECT domain-containing proteins function as ubiquitin-protein ligases, or E3 enzymes. EDD has been shown to bind to ubiquitin, and like other HECT family proteins, may function as an E3 ubiquitin-protein ligase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CH D1S3362; E3 identified by differential display; E3 ubiquitin-protein ligase UBR5; E3 ubiquitin-protein ligase, HECT domain-containing 1; ECGF1; EDG-1; EDG1; extraembryonic development; HECT E3; ubiquitin-protein ligase; HECT-type E3 ubiquitin transferase UBR5; hHYD; Hyperplastic discs protein homolog; Progestin-induced protein; S1P receptor 1; S1P receptor Edg-1; S1P1; S1PR1; unnamed protein product
Gene Aliases: DD5; EDD; EDD1; HYD; KIAA0896; UBR5
UniProt ID: (Human) O95071, (Mouse) Q698K9
Entrez Gene ID: (Human) 51366, (Mouse) 70790
RNA binding
ubiquitin-protein transferase activity
protein binding
zinc ion binding
transferase activity
ubiquitin-ubiquitin ligase activity
ubiquitin binding
metal ion binding
ubiquitin protein ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
protein modifying enzyme
protein polyubiquitination
DNA repair
cellular response to DNA damage stimulus
response to oxidative stress
proteasomal protein catabolic process
positive regulation of gene expression
protein ubiquitination
intracellular estrogen receptor signaling pathway
negative regulation of extent of heterochromatin assembly
protein K29-linked ubiquitination
positive regulation of protein import into nucleus
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of smoothened signaling pathway
retinoic acid receptor signaling pathway
progesterone receptor signaling pathway
vitamin D receptor signaling pathway
protein K48-linked ubiquitination
protein K11-linked ubiquitination
cytoplasm-associated proteasomal ubiquitin-dependent protein catabolic process
nucleus-associated proteasomal ubiquitin-dependent protein catabolic process
positive regulation of canonical Wnt signaling pathway
cytoplasm protein quality control
DNA repair-dependent chromatin remodeling
protein branched polyubiquitination
positive regulation of protein import into nucleus, translocation
positive regulation of catenin import into nucleus
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of histone H2A K63-linked ubiquitination
negative regulation of double-strand break repair
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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