Immunohistochemical analysis of EDF1 in paraffin-embedded human spleen following steamed antigen retrieval with a citrate buffer, pH 6, using an EDF1 polyclonal antibody (Product# PA5-37908) at a dilution of 5ug/ml. Detection was performed using an AP-conjugated secondary antibody.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence C-NKQHSITKNTAKLDR, from the internal region of EDF1|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5-10 µg/ml|
|Western Blot (WB)||0.3-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with mouse, rat and pig based on sequence homology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.