Proteintech

EEF1A1 Polyclonal Antibody

EEF1A1 Antibody in Immunocytochemistry (ICC/IF)

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EEF1A1 Antibody (11402-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF1A1 antibody (Product # 11402-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

EEF1A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

11402-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 3 publications 3 publications

Immunoprecipitation (IP)

View 2 publications 2 publications

RNA Immunoprecipitation (RIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

EEF1A1 Fusion Protein Ag1938 (144-462 aa encoded by BC014224)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.32 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human EEF1A1.

Immunogen sequence: GVKQLIVGV NKMDSTEPPY SQKRYEEIVK EVSTYIKKIG YNPDTVAFVP ISGWNGDNML EPSANMPWFK GWKVTRKDGN ASGTTLLEAL DCILPPTRPT DKPLRLPLQD VYKIGGIGTV PVGRVETGVL KPGMVVTFAP VNVTTEVKSV EMHHEALSEA LPGDNVGFNV KNVSVKDVRR GNVAGDSKND PPMEAAGFTA QVIILNHPGQ ISAGYAPVLD CHTAHIACKF AELKEKIDRR SGKKLEDGPK FLKSGDAAIV DMVPGKPMCV ESFSDYPPLG RFAVRDMRQT VAVGVIKAVD KKAAGAGKVT KSAQKAQKAK (144-462 aa encoded by BC014224)

Target Information

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Epitranscriptic regulation of HRAS by N⁶-methyladenosine drives tumor progression.

Proceedings of the National Academy of Sciences of the United States of America

Pan Y,Gu Y,Liu T,Zhang Q,Yang F,Duan L,Cheng S,Zhu X,Xi Y,Chang X,Ye Q,Gao S

Tue Apr 04 00:00:00 UTC 2023

Anisomycin inhibits Coxsackievirus B replication by promoting the lysosomal degradation of eEF1A1.

Antiviral research

Shao E,Zhao S,Dong Y,Wang Y,Fei Y,Li S,Wang L,Bashir T,Luan T,Lin L,Wang Y,Zhao W,Zhong Z

Sat Jul 01 00:00:00 UTC 2023

A photoaffinity labeling strategy identified EF1A1 as a binding protein of cyclic dinucleotide 2'3'-cGAMP.

Cell chemical biology

Hou Y,Lu H,Li J,Guan Z,Zhang J,Zhang W,Yin C,Sun L,Zhang Y,Jiang H

Thu Jan 20 00:00:00 UTC 2022

N⁶-methyladenosine modification regulates imatinib resistance of gastrointestinal stromal tumor by enhancing the expression of multidrug transporter MRP1.

Cancer letters

Xu K,Zhang Q,Chen M,Li B,Wang N,Li C,Gao Z,Zhang D,Yang L,Xu Z,Li X,Xu H

Fri Apr 01 00:00:00 UTC 2022

N⁶-methyladenosine modification regulates imatinib resistance of gastrointestinal stromal tumor by enhancing the expression of multidrug transporter MRP1.

Cancer letters

Xu K,Zhang Q,Chen M,Li B,Wang N,Li C,Gao Z,Zhang D,Yang L,Xu Z,Li X,Xu H

Fri Apr 01 00:00:00 UTC 2022

Cytosolic PINK1 orchestrates protein translation during proteasomal stress by phosphorylating the translation elongation factor eEF1A1.

FEBS letters

Qin S,Ye L,Zheng Y,Gao J

Mon Feb 01 00:00:00 UTC 2021

Cytosolic PINK1 orchestrates protein translation during proteasomal stress by phosphorylating the translation elongation factor eEF1A1.

FEBS letters

Qin S,Ye L,Zheng Y,Gao J

Mon Feb 01 00:00:00 UTC 2021

CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome.

Science advances

Asamitsu S,Yabuki Y,Ikenoshita S,Kawakubo K,Kawasaki M,Usuki S,Nakayama Y,Adachi K,Kugoh H,Ishii K,Matsuura T,Nanba E,Sugiyama H,Fukunaga K,Shioda N

Fri Jan 01 00:00:00 UTC 2021

Opposing roles of C/EBPα and eEF1A1 in Sp1-regulated miR-122 transcription.

RNA biology

Zeng C,Sang Y,Wang FY,Zhuang SM

Sat Feb 01 00:00:00 UTC 2020

Accumulation of EBI3 induced by virulent Mycobacterium tuberculosis inhibits apoptosis in murine macrophages.

Pathogens and disease

Deng JH,Chen HY,Huang C,Yan JM,Yin Z,Zhang XL,Pan Q

Fri Feb 01 00:00:00 UTC 2019

A rapid and specific method to simultaneously quantify eukaryotic elongation factor 1A1 and A2 protein levels in cancer cells.

Journal of pharmaceutical and biomedical analysis

Bosutti A,Kalaja O,Zanconati F,Dapas B,Grassi G,Passamonti S,Scaggiante B

Sat Nov 30 00:00:00 UTC 2019

A rapid and specific method to simultaneously quantify eukaryotic elongation factor 1A1 and A2 protein levels in cancer cells.

Journal of pharmaceutical and biomedical analysis

Bosutti A,Kalaja O,Zanconati F,Dapas B,Grassi G,Passamonti S,Scaggiante B

Sat Nov 30 00:00:00 UTC 2019

A rapid and specific method to simultaneously quantify eukaryotic elongation factor 1A1 and A2 protein levels in cancer cells.

Journal of pharmaceutical and biomedical analysis

Bosutti A,Kalaja O,Zanconati F,Dapas B,Grassi G,Passamonti S,Scaggiante B

Sat Nov 30 00:00:00 UTC 2019

Novel nannocystin A analogues as anticancer therapeutics: Synthesis, biological evaluations and structure-activity relationship studies.

European journal of medicinal chemistry

Liu Q,Yang X,Ji J,Zhang SL,He Y

Wed May 15 00:00:00 UTC 2019

RNA m⁶A methylation regulates the epithelial mesenchymal transition of cancer cells and translation of Snail.

Nature communications

Lin X,Chai G,Wu Y,Li J,Chen F,Liu J,Luo G,Tauler J,Du J,Lin S,He C,Wang H

Mon May 06 00:00:00 UTC 2019

Acetylation of Cytidine in mRNA Promotes Translation Efficiency.

Cell

Arango D,Sturgill D,Alhusaini N,Dillman AA,Sweet TJ,Hanson G,Hosogane M,Sinclair WR,Nanan KK,Mandler MD,Fox SD,Zengeya TT,Andresson T,Meier JL,Coller J,Oberdoerffer S

Thu Dec 13 00:00:00 UTC 2018

Acetylation of Cytidine in mRNA Promotes Translation Efficiency.

Cell

Arango D,Sturgill D,Alhusaini N,Dillman AA,Sweet TJ,Hanson G,Hosogane M,Sinclair WR,Nanan KK,Mandler MD,Fox SD,Zengeya TT,Andresson T,Meier JL,Coller J,Oberdoerffer S

Thu Dec 13 00:00:00 UTC 2018

Contradictory mRNA and protein misexpression of EEF1A1 in ductal breast carcinoma due to cell cycle regulation and cellular stress.

Scientific reports

Lin CY,Beattie A,Baradaran B,Dray E,Duijf PHG

Mon Sep 17 00:00:00 UTC 2018

Contradictory mRNA and protein misexpression of EEF1A1 in ductal breast carcinoma due to cell cycle regulation and cellular stress.

Scientific reports

Lin CY,Beattie A,Baradaran B,Dray E,Duijf PHG

Mon Sep 17 00:00:00 UTC 2018

Single-cell gene expression analysis reveals diversity among human spermatogonia.

Molecular human reproduction

Neuhaus N,Yoon J,Terwort N,Kliesch S,Seggewiss J,Huge A,Voss R,Schlatt S,Grindberg RV,Schöler HR

Fri Feb 10 00:00:00 UTC 2017

Single-cell gene expression analysis reveals diversity among human spermatogonia.

Molecular human reproduction

Neuhaus N,Yoon J,Terwort N,Kliesch S,Seggewiss J,Huge A,Voss R,Schlatt S,Grindberg RV,Schöler HR

Fri Feb 10 00:00:00 UTC 2017

The Ubiquitin-like Protein FAT10 Stabilizes eEF1A1 Expression to Promote Tumor Proliferation in a Complex Manner.

Cancer research

Liu X,Chen L,Ge J,Yan C,Huang Z,Hu J,Wen C,Li M,Huang D,Qiu Y,Hao H,Yuan R,Lei J,Yu X,Shao J

Mon Aug 15 00:00:00 UTC 2016

The Ubiquitin-like Protein FAT10 Stabilizes eEF1A1 Expression to Promote Tumor Proliferation in a Complex Manner.

Cancer research

Liu X,Chen L,Ge J,Yan C,Huang Z,Hu J,Wen C,Li M,Huang D,Qiu Y,Hao H,Yuan R,Lei J,Yu X,Shao J

Mon Aug 15 00:00:00 UTC 2016

The Ubiquitin-like Protein FAT10 Stabilizes eEF1A1 Expression to Promote Tumor Proliferation in a Complex Manner.

Cancer research

Liu X,Chen L,Ge J,Yan C,Huang Z,Hu J,Wen C,Li M,Huang D,Qiu Y,Hao H,Yuan R,Lei J,Yu X,Shao J

Mon Aug 15 00:00:00 UTC 2016

The Ubiquitin-like Protein FAT10 Stabilizes eEF1A1 Expression to Promote Tumor Proliferation in a Complex Manner.

Cancer research

Liu X,Chen L,Ge J,Yan C,Huang Z,Hu J,Wen C,Li M,Huang D,Qiu Y,Hao H,Yuan R,Lei J,Yu X,Shao J

Mon Aug 15 00:00:00 UTC 2016

Bioinformatics analysis of proteomics profiles in senescent human primary proximal tubule epithelial cells.

BMC nephrology

Lu Y,Wang J,Dapeng C,Wu D,Cai G,Chen X

Fri Apr 01 00:00:00 UTC 2016

Bioinformatics analysis of proteomics profiles in senescent human primary proximal tubule epithelial cells.

BMC nephrology

Lu Y,Wang J,Dapeng C,Wu D,Cai G,Chen X

Fri Apr 01 00:00:00 UTC 2016

Identification of differentially expressed genes in human cryptorchid testes using suppression subtractive hybridization.

The Journal of urology

Mizuno K,Kojima Y,Kurokawa S,Maruyama T,Sasaki S,Kohri K,Hayashi Y

Sun Mar 01 00:00:00 UTC 2009

Identification of differentially expressed genes in human cryptorchid testes using suppression subtractive hybridization.

The Journal of urology

Mizuno K,Kojima Y,Kurokawa S,Maruyama T,Sasaki S,Kohri K,Hayashi Y

Sun Mar 01 00:00:00 UTC 2009

Identification of differentially expressed genes in human cryptorchid testes using suppression subtractive hybridization.

The Journal of urology

Mizuno K,Kojima Y,Kurokawa S,Maruyama T,Sasaki S,Kohri K,Hayashi Y

Sun Mar 01 00:00:00 UTC 2009

Identification of differentially expressed genes in human cryptorchid testes using suppression subtractive hybridization.

The Journal of urology

Mizuno K,Kojima Y,Kurokawa S,Maruyama T,Sasaki S,Kohri K,Hayashi Y

Sun Mar 01 00:00:00 UTC 2009

Quantitative proteomic analysis of a paired human liver healthy versus carcinoma cell lines with the same genetic background to identify potential hepatocellular carcinoma markers.

Proteomics. Clinical applications

Bao H,Song P,Liu Q,Liu Y,Yun D,Saiyin H,Du R,Zhang Y,Fan H,Yang P,Chen X

Mon Jun 01 00:00:00 UTC 2009

Quantitative proteomic analysis of a paired human liver healthy versus carcinoma cell lines with the same genetic background to identify potential hepatocellular carcinoma markers.

Proteomics. Clinical applications

Bao H,Song P,Liu Q,Liu Y,Yun D,Saiyin H,Du R,Zhang Y,Fan H,Yang P,Chen X

Mon Jun 01 00:00:00 UTC 2009

Quantitative proteomic analysis of a paired human liver healthy versus carcinoma cell lines with the same genetic background to identify potential hepatocellular carcinoma markers.

Proteomics. Clinical applications

Bao H,Song P,Liu Q,Liu Y,Yun D,Saiyin H,Du R,Zhang Y,Fan H,Yang P,Chen X

Mon Jun 01 00:00:00 UTC 2009

Quantitative proteomic analysis of a paired human liver healthy versus carcinoma cell lines with the same genetic background to identify potential hepatocellular carcinoma markers.

Proteomics. Clinical applications

Bao H,Song P,Liu Q,Liu Y,Yun D,Saiyin H,Du R,Zhang Y,Fan H,Yang P,Chen X

Mon Jun 01 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: cervical cancer suppressor 3; CTCL tumor antigen; eEF1A-1; EF-1 alpha2; EF-1-alpha-1; EF-Tu; EF1a-like protein; EF1A1; elongation factor 1 A-1; elongation factor 1 alpha subunit; Elongation factor 1-alpha 1; Elongation factor Tu; Eukaryotic elongation factor 1 A-1; eukaryotic translation elongation factor 1 alpha 1-like 14; eukaryotic translation elongation factor 1 alpha 2 like 1; glucocorticoid receptor AF-1 specific elongation factor; GRAF 1EF; leukocyte receptor cluster (LRC) member 7; Leukocyte receptor cluster member 7; prostate tumor-inducing protein 1; translation elongation factor 1 alpha 1-like 14

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Gene Aliases: CCS-3; CCS3; EE1A1; EEF-1; EEF1A; eEF1A-1; EEF1A1; Eef1a2; Eef1a2l1; EF-Tu; EF1A; GRAF-1EF; HNGC:16303; LENG7; PTI1; SI

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UniProt ID: (Human) P68104, (Mouse) P10126, (Rat) P62630

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Entrez Gene ID: (Human) 1915, (Mouse) 13627, (Rat) 171361

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Function(s)

tRNA binding translation elongation factor activity GTPase activity protein binding GTP binding protein kinase binding poly(A) RNA binding nucleotide binding mRNA binding translation factor

Process(es)

transcription, DNA-templated regulation of transcription, DNA-templated translational elongation cellular response to epidermal growth factor stimulus regulation of chaperone-mediated autophagy translation protein homooligomerization positive regulation of apoptotic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EEF1A1 Polyclonal Antibody

EEF1A1 Antibody (11402-1-AP) in ICC/IF

Product Details

11402-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

RNA Immunoprecipitation (RIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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