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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500-1:10,000 | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:100-1:1,000 | - |
Immunocytochemistry (ICC/IF) |
1:100-1:1,000 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Recombinant fragment corresponding to a region within amino acids 1 and 268 of EEF2K (Uniprot ID#O00418) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
0.1M tris glycine, pH 7, with 10% glycerol |
Contains |
0.01% thimerosal |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_11153234 |
Recommended positive controls: A431, H1299, HeLa, HepG2, Raji, mouse liver, PC-12.
Predicted reactivity: Mouse (91%), Rat (89%), Bovine (90%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
EEF2K (eukaryotic elongation factor 2 kinase) is a threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Up activation, by a variety of upstream kinases including AMPK or TRPM7, EEF2K phosphorylates the elongation factor EEF2 at a single site. Then, EEF2K renders EEF2 unable to bind ribosomes and inactive - in turn, the rate of protein synthesis is reduced.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase; calmodulin-dependent protein kinase III; EC 2.7.11.20; eEF-2 kinase; eEF-2K; EF2K; elongation factor 2 kinase; elongation factor-2 kinase; euk; eukaroytic elongation factor 2 kinase; Eukaryotic elongation factor 2 kinase; eukaryotic elongation factor-2 kinase; kinase eEF2K
Gene Aliases: C86191; eEF-2K; EEF2K; HSU93850; SMEF2K
UniProt ID: (Human) O00418, (Mouse) O08796, (Rat) P70531
Entrez Gene ID: (Human) 29904, (Mouse) 13631, (Rat) 25435
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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