Invitrogen

EEF2K Polyclonal Antibody

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EEF2K Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of EEF2K in paraformaldehyde-fixed A431 cells using an EEF2K polyclonal antibody (Product # PA5-22175) at a 1:200 dilution.

EEF2K Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of EEF2K in paraffin-embedded BT483 xenograft using an EEF2K polyclonal antibody (Product # PA5-22175) at a dilution of 1:500.

EEF2K Antibody in Western Blot (WB) — Western Blot analysis of EEF2K was performed by separating 30 µg of Whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a EEF2K Polyclonal Antibody (Product # PA5-22175) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Western Blot (WB)

1:500-1:10,000

Product Specifications

Species

Human, Mouse, Rat

Expression System

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 268 of EEF2K (Uniprot ID#O00418)

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_11153234

Product Specific Information

Recommended positive controls: A431, H1299, HeLa, HepG2, Raji, mouse liver, PC-12.

Predicted reactivity: Mouse (91%), Rat (89%), Bovine (90%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

EEF2K (eukaryotic elongation factor 2 kinase) is a threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Up activation, by a variety of upstream kinases including AMPK or TRPM7, EEF2K phosphorylates the elongation factor EEF2 at a single site. Then, EEF2K renders EEF2 unable to bind ribosomes and inactive - in turn, the rate of protein synthesis is reduced.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase; calmodulin-dependent protein kinase III; EC 2.7.11.20; eEF-2 kinase; eEF-2K; EF2K; elongation factor 2 kinase; elongation factor-2 kinase; euk; eukaroytic elongation factor 2 kinase; Eukaryotic elongation factor 2 kinase; eukaryotic elongation factor-2 kinase; kinase eEF2K

Gene Aliases: C86191; eEF-2K; EEF2K; HSU93850; SMEF2K

UniProt ID: (Human) O00418, (Mouse) O08796, (Rat) P70531

Entrez Gene ID: (Human) 29904, (Mouse) 13631, (Rat) 25435

Molecular Function: Hsp70 family chaperone chaperone kinase non-receptor serine/threonine protein kinase protein kinase transferase