|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the carboxy terminal region of human EGFR.|
|Storage buffer||PBS with 0.2% gelatin|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100 - 1:200|
|Immunoprecipitation (IP)||1-2 µg/ml|
|Western Blot (WB)||1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
OPA1-10100 detects EGFR from human, mouse and rat samples.
OPA1-10100 has been successfully used in Western blot, immunohistochemistry and immunoprecipitation procedures.
OPA1-10100 immunizing peptide corresponds to the carboxy terminal region of human EGFR.
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Inhibition of HER-2/neu kinase impairs androgen receptor recruitment to the androgen responsive enhancer.
OPA1-10100 was used in immunoprecipitation to investigate the role of Her-2/neu kinase in the androgen receptor signalling transduction pathway
|Liu Y,Majumder S,McCall W,Sartor CI,Mohler JL,Gregory CW,Earp HS,Whang YE||Cancer research (65:3404)||2005|