Immunofluorescent analysis of EGFR (green) in untreated HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product #31873) for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing EGFR (Product # PA1-1110), at a dilution of 1:500 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Rat, Non-human primate, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C L(1198) R V A P Q S S E F I G A(1210) of human EGFR.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2 µg|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-1110 detects EGFR from human, non-human primate (monkey), mouse, and rat samples.
PA1-1110 has been successfully used in Western blot, immunoprecipitation, immunohistochemistry, and immunofluorescence procedures. By Western blot, this antibody detects ~190 kDa protein representing EGFR from human HeLa cell extract.
The PA1-1110 immunogen is a synthetic peptide corresponding to residues C L(1198) R V A P Q S S E F I G A(1210) of human EGFR.
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.
IP-MS enrichment of EGFR (LFQ intensity): EGFR was enriched 152-fold from A549 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and EGFR antibody (Part No. PA1-1110). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Establishment and characterization of rat portal myofibroblast cell lines.
PA1-1110 was used in western blot to characterize two new cell lines with the potential to be used to study portal (myo)fibroblasts
|Fausther M,Goree JR,Lavoie ÉG,Graham AL,Sévigny J,Dranoff JA||PloS one (10:null)||2015|
A genetically encoded alkyne directs palladium-mediated protein labeling on live mammalian cell surface.
PA1-1110 was used in western blot to show that a new alkyne amino acid can be site-selectively incorporated into mammalian proteins
|Li N,Ramil CP,Lim RK,Lin Q||ACS chemical biology (10:379)||2015|
|Non-human primate||Not Cited||
NT5E mutations that cause human disease are associated with intracellular mistrafficking of NT5E protein.
PA1-1110 was used in western blot to study the association of disease-causing NT5E mutations with intracellular NT5E mistrafficking
|Fausther M,Lavoie EG,Goree JR,Baldini G,Dranoff JA||PloS one (9:null)||2014|
Targeting RNA foci in iPSC-derived motor neurons from ALS patients with a C9ORF72 repeat expansion.
PA1-1110 was used in western blot to study the accumulation of RNA foci in motor neurons derived from induced pluripotent stem cells obtained from a patient with C9ORF72 repeat ALS
|Sareen D,O'Rourke JG,Meera P,Muhammad AK,Grant S,Simpkinson M,Bell S,Carmona S,Ornelas L,Sahabian A,Gendron T,Petrucelli L,Baughn M,Ravits J,Harms MB,Rigo F,Bennett CF,Otis TS,Svendsen CN,Baloh RH||Science translational medicine (5:null)||2013|
Cell-free reconstitution of multivesicular body formation and receptor sorting.
PA1-1110 was used in western blot to develop a novel cell-free assay to study multivesicular body formation and receptor sorting
|Sun W,Vida TA,Sirisaengtaksin N,Merrill SA,Hanson PI,Bean AJ||Traffic (Copenhagen, Denmark) (11:867)||2010|
The UIM domain of Hrs couples receptor sorting to vesicle formation.
PA1-1110 was used in immunocytochemistry to investigate the roles of the UIM-domain of Hrs in the sorting of EGFR and vesicle formation
|Urbé S,Sachse M,Row PE,Preisinger C,Barr FA,Strous G,Klumperman J,Clague MJ||Journal of cell science (116:4169)||2003|