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Invitrogen
EGR1 Polyclonal Antibody
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FIGURE: 1 / 1
EGR1 Antibody (PA5-80574) in ICC/IF
Immunofluorescence staining of EGR1 in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and then incubated with EGR1 Polyclonal Antibody (Product # PA5-80574, 1:1,000)(Figure A) or EGR1 Polyclonal Antibody and antigen (Figure B) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.
Product Details
PA5-80574
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human EGR1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human EGR1.
Target Information
The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AT225; Early growth response protein 1; EGR-1; ETR103; Nerve growth factor-induced protein A; NGFI-A; Transcription factor ETR103; Transcription factor Zif268; unnamed protein product; zinc finger gene 225; Zinc finger protein 225; Zinc finger protein Krox-24
Gene Aliases: AT225; EGR1; G0S30; KROX-24; KROX24; NGFI-A; TIS8; ZIF-268; ZIF268; ZNF225
UniProt ID: (Human) P18146
Entrez Gene ID: (Human) 1958
transcription regulatory region sequence-specific DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
zinc ion binding
double-stranded methylated DNA binding
enzyme binding
histone acetyltransferase binding
sequence-specific DNA binding
hemi-methylated DNA-binding
metal ion binding
sequence-specific double-stranded DNA binding
promoter-specific chromatin binding
negative regulation of transcription from RNA polymerase II promoter
response to hypoxia
response to ischemia
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
response to glucose
positive regulation of gene expression
T cell differentiation
BMP signaling pathway
positive regulation of chemokine production
positive regulation of interleukin-1 beta production
response to insulin
circadian regulation of gene expression
regulation of protein sumoylation
skeletal muscle cell differentiation
regulation of apoptotic process
regulation of neuron apoptotic process
hypomethylation of CpG island
estrous cycle
locomotor rhythm
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of hormone biosynthetic process
rhythmic process
circadian temperature homeostasis
interleukin-1-mediated signaling pathway
cellular response to gamma radiation
cellular response to heparin
cellular response to mycophenolic acid
glomerular mesangial cell proliferation
positive regulation of glomerular metanephric mesangial cell proliferation
negative regulation of canonical Wnt signaling pathway
cellular response to interleukin-8
positive regulation of post-translational protein modification
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
regulation of progesterone biosynthetic process
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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