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Invitrogen
EHD2 Polyclonal Antibody
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FIGURE: 1 / 1
EHD2 Antibody (PA5-116554) in WB
Western blot analysis of EHD2 in extracts of various cell lines. Samples were incubated with EHD2 Polyclonal antibody (Product # PA5-116554) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-116554
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 244-543 of human EHD2 (NP_0554162)
Conjugate
Form
Liquid
Concentration
0.05 mg/mL
Amount
5 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: A-549, U-87MG, HeLa, Mouse lung, Rat lung
Immunogen sequence: VGTPEVLRVY IGSFWSQPLL VPDNRRLFEL EEQDLFRDIQ GLPRHAALRK LNDLVKRARL VRVHAYIISY LKKEMPSVFG KENKKKQLIL KLPVIFAKIQ LEHHISPGDF PDCQKMQELL MAHDFTKFHS LKPKLLEALD EMLTHDIAKL MPLLRQEELE STEVGVQGGA FEGTHMGPFV ERGPDEAMED GEEGSDDEAE WVVTKDKSKY DEIFYNLAPA DGKLSGSKAK TWMVGTKLPN SVLGRIWKLS DVDRDGMLDD EEFALASHLI EAKLEGHGLP ANLPRRLVPP SKRRHKGSAE
Target Information
This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EH domain-containing protein 2; EHD2; FLJ96617; PAST homolog 2; putative eps protein; similar to EHD1; unnamed protein product
Gene Aliases: BC027084; C130052H20Rik; EHD2; MGEPS; PAST2
UniProt ID: (Human) Q9NZN4, (Rat) Q4V8H8, (Mouse) Q8BH64
Entrez Gene ID: (Human) 30846, (Rat) 361512, (Mouse) 259300
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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