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          • Primary Antibodies ›
          • EHD2 Antibodies

          Invitrogen

          EHD2 Polyclonal Antibody

          1 Reference
          View all (10) EHD2 antibodies

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          Cite EHD2 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (3)
          EHD2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          EHD2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          EHD2 Antibody (PA5-80576) in ICC/IF

          Immunofluorescence staining of EHD2 in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with EHD2 Polyclonal Antibody (Product # PA5-80576, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          EHD2 Antibody in Immunocytochemistry (ICC/IF)
          EHD2 Antibody in Western Blot (WB)
          EHD2 Antibody in Immunoprecipitation (IP)
          EHD2 Polyclonal Antibody

          Product Details

          PA5-80576

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:300-1:10,000
          -

          Immunoprecipitation (IP)

          1-4 µL/mg of lysate
          -
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthetic peptide corresponding to the C-terminus of the Human EHD2
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Protein A, Antigen affinity chromatography

          Storage buffer

          PBS

          Contains

          no preservative

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2787875

          Product Specific Information

          This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

          This antibody has specificity for Human EHD2.

          Target Information

          This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: EH domain-containing protein 2; EHD2; FLJ96617; PAST homolog 2; similar to EHD1; unnamed protein product

          View more View less

          Gene Aliases: EHD2; PAST2

          View more View less

          UniProt ID: (Human) Q9NZN4

          View more View less

          Entrez Gene ID: (Human) 30846

          View more View less

          Function(s)
          nucleotide binding nucleic acid binding calcium ion binding protein binding ATP binding GTP binding hydrolase activity protein domain specific binding protein binding, bridging identical protein binding metal ion binding
          Process(es)
          endocytosis cortical actin cytoskeleton organization endocytic recycling cilium morphogenesis protein localization to plasma membrane membrane tubulation positive regulation of myoblast fusion positive regulation of endocytic recycling
          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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