Invitrogen

EHD3 Polyclonal Antibody

View all (9) EHD3 antibodies

Questions & Answers

Questions & Answers

Product Image Gallery

EHD3 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis in formalin-fixed, paraffin-embedded brain tissue using an EHD3 polyclonal antibody (Product # PA5-25963), followed by HRP-conjugated secondary antibody and DAB staining.

EHD3 Antibody in Western Blot (WB) — Western blot analysis using an EHD3 polyclonal antibody (Product # PA5-25963) in mouse lung tissue lysates (35 µg per lane).

EHD3 Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of HepG2 cells using an EHD3 polyclonal antibody (Product # PA5-25963) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Product Details

PA5-25963

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:10-1:50	-
Flow Cytometry (Flow)	1:10-1:50	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KLH conjugated synthetic peptide between 22-48 amino acids from the N-terminal region of human EHD3 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	2 mg/mL
Purification	Ammonium sulfate precipitation, Size-exclusion - Dialysis
Storage buffer	PBS
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2543463

Product Specific Information

This antibody is predicted to react with bovine, mouse and rat based on sequence homology.

Target Information

EHD3 plays a role in endocytic transport.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: EH domain-containing protein 3; EH-domain containing 3; EH-domain containing protein 2; PAST homolog 3

Gene Aliases: EHD2; EHD3; PAST3

UniProt ID: (Human) Q9NZN3, (Mouse) Q9QXY6

Entrez Gene ID: (Human) 30845, (Mouse) 57440

Molecular Function: membrane traffic protein

receptor recycling blood coagulation endocytic recycling early endosome to Golgi transport cilium assembly protein homooligomerization protein targeting to plasma membrane regulation of cardiac muscle cell membrane potential Golgi to lysosome transport regulation of Golgi organization

Process(es)

nucleic acid binding calcium ion binding protein binding ATP binding GTP binding nucleotide binding metal ion binding