Invitrogen

EHHADH Polyclonal Antibody

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EHHADH Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ECHD in A549 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with ECHD polyclonal antibody (Product # PA5-101741) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

EHHADH Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded ECHD in mouse liver tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with ECHD polyclonal antibody (Product # PA5-101741) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

EHHADH Antibody in Western Blot (WB) — Western blot analysis of ECHD in A549 cell lysate (left lane: treated with the antigen-specific peptide). Samples were incubated with ECHD polyclonal antibody (Product # PA5-101741).

Product Details

PA5-101741

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human EHHADH(Accession Q08426), corresponding to amino acid residues L485-F512. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2851174

Target Information

As an L-hydroxy-specific enzyme, EHHADH (enoyl-CoA-hydratase:3-hydroxyacyl-CoA dehydrogenase), also known as Peroxisomal L-bifunctional enzyme, is a 723 amino acid protein has an essential tripeptide sequence on its carboxyl-terminus that is required for peroxisomal transport. EHHADH-null mice only exhibit a blunted peroxisome proliferative response when challenged with a peroxisome proliferator. Since there were no observed changes in lipid metabolism, this evidence suggests that enoyl-CoAs were diverted to the D-hydroxy-specific beta-oxidation system for metabolism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 3,2-trans-enoyl-CoA isomerase; 3-hydroxyacyl-CoA dehydrogenase; Enoyl-CoA hydratase/3,2-trans-enoyl-CoA isomerase; enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase; L-3-hydroxyacyl-CoA dehydrogenase; L-bifunctional enzyme; L-bifunctional protein; L-bifunctional protein, peroxisomal; L-PBE; L-peroxisomal bifunctional enzyme; L-specific bifunctional protein; L-specific multifunctional beta-oxdiation protein; LBP; MFE1; MFP-1; MFP1; Multifunctional enzyme 1; multifunctional enzyme type 1; Multifunctional protein 1; PBE; Peroxisomal bifunctional enzyme; peroxisomal bifunctional enzyme type 1; peroxisomal enoyl-CoA hydratase

Gene Aliases: ECHD; EHHADH; HD; LBFP; LBP; MEF; Mfe; MFP; Pbe; PBFE; 1; 1300002P22Rik; FRTS3; L-PBE; Mfe1; MFP1; perMFE-1

UniProt ID: (Human) Q08426, (Mouse) Q9DBM2, (Rat) P07896

Entrez Gene ID: (Human) 1962, (Mouse) 74147, (Rat) 171142

Molecular Function: dehydrogenase