Invitrogen

EHHADH Polyclonal Antibody

Product Image Gallery

EHHADH Antibody in Western Blot (WB) — Western blot analysis of ECHD in A549 cell lysate (left lane: treated with the antigen-specific peptide). Samples were incubated with ECHD polyclonal antibody (Product # PA5-101741).

EHHADH Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded ECHD in mouse liver tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with ECHD polyclonal antibody (Product # PA5-101741) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

EHHADH Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of ECHD in A549 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with ECHD polyclonal antibody (Product # PA5-101741) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC)

1:100-1:500

Immunofluorescence (IF)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human EHHADH, corresponding to a region within the internal amino acids.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol, 150mM NaCl

Contains

0.02% sodium azide

Storage conditions

-20°C

RRID

AB_2851174

Target Information

As an L-hydroxy-specific enzyme, EHHADH (enoyl-CoA-hydratase:3-hydroxyacyl-CoA dehydrogenase), also known as Peroxisomal L-bifunctional enzyme, is a 723 amino acid protein has an essential tripeptide sequence on its carboxyl-terminus that is required for peroxisomal transport. EHHADH-null mice only exhibit a blunted peroxisome proliferative response when challenged with a peroxisome proliferator. Since there were no observed changes in lipid metabolism, this evidence suggests that enoyl-CoAs were diverted to the D-hydroxy-specific beta-oxidation system for metabolism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: 3,2-trans-enoyl-CoA isomerase; 3-hydroxyacyl-CoA dehydrogenase; Enoyl-CoA hydratase/3,2-trans-enoyl-CoA isomerase; enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase; L-3-hydroxyacyl-CoA dehydrogenase; L-bifunctional enzyme; L-bifunctional protein, peroxisomal; L-specific bifunctional protein; L-specific multifunctional beta-oxdiation protein; multifunctional enzyme type 1; PBE; Peroxisomal bifunctional enzyme; peroxisomal bifunctional enzyme type 1; peroxisomal enoyl-CoA hydratase

Gene Aliases: ECHD; EHHADH; HD; LBFP; LBP; MEF; Mfe; MFP; Pbe; PBFE; 1; 1300002P22Rik; FRTS3; L-PBE; Mfe1; MFP1; perMFE-1

UniProt ID: (Human) Q08426, (Mouse) Q9DBM2, (Rat) P07896

Entrez Gene ID: (Human) 1962, (Mouse) 74147, (Rat) 171142

Molecular Function: dehydrogenase epimerase/racemase hydratase isomerase lyase oxidoreductase