Invitrogen

EHHADH Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (13) EHHADH antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

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EHHADH Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of ECHD in human liver and cerebral cortex tissues using a ECHD polyclonal antibody (Product # PA5-83586). Corresponding RNA-seq data are presented for the same tissues.

EHHADH Antibody in Relative expression — Relative expression in different tissues in IHC: Detection of differential expression levels of ECHD demonstrates antibody specificity. Immunohistochemical analysis of ECHD using anti-ECHD Polyclonal Antibody (Product #PA5-83586), shows significant staining of ECHD in liver and shows minimal or weak staining in cerebral cortex tissues. The relative expression levels of ECHD within each tissue is shown using RNA-Seq. {RE}

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human EHHADH

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2790739

Product Specific Information

Immunogen sequence: ILGEGIAASP EHIDVVYLHG YGWPRHKGGP MFYASTVGLP TVLEKLQKYY RQNPDIPQLE PSDYLKKLAS QGNPPLKE

Target Information

As an L-hydroxy-specific enzyme, EHHADH (enoyl-CoA-hydratase:3-hydroxyacyl-CoA dehydrogenase), also known as Peroxisomal L-bifunctional enzyme, is a 723 amino acid protein has an essential tripeptide sequence on its carboxyl-terminus that is required for peroxisomal transport. EHHADH-null mice only exhibit a blunted peroxisome proliferative response when challenged with a peroxisome proliferator. Since there were no observed changes in lipid metabolism, this evidence suggests that enoyl-CoAs were diverted to the D-hydroxy-specific beta-oxidation system for metabolism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 3,2-trans-enoyl-CoA isomerase; 3-hydroxyacyl-CoA dehydrogenase; Enoyl-CoA hydratase/3,2-trans-enoyl-CoA isomerase; enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase; L-3-hydroxyacyl-CoA dehydrogenase; L-bifunctional protein, peroxisomal; PBE; Peroxisomal bifunctional enzyme; peroxisomal enoyl-CoA hydratase

Gene Aliases: ECHD; EHHADH; FRTS3; L-PBE; LBFP; LBP; PBFE

UniProt ID: (Human) Q08426

Entrez Gene ID: (Human) 1962

Molecular Function: dehydrogenase epimerase/racemase hydratase isomerase lyase oxidoreductase