Proteintech

EIF2AK2/PKR Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

EIF2AK2/PKR Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of MCF-7 cells, using EIF2AK2 antibody 18244-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

EIF2AK2/PKR Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human testis tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

EIF2AK2/PKR Antibody in Western Blot (WB) — mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

EIF2AK2/PKR Antibody in Immunoprecipitation (IP) — IP result of anti-EIF2AK2, PKR (IP:18244-1-AP, 4ug; Detection:18244-1-AP 1:500) with HeLa cells lysate 1200ug.

Product Details

18244-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:4,000	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EIF2AK2, PKR Fusion Protein Ag13010 (1-373 aa encoded by BC093676) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.21 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody is specific to EIF2AK2.

Immunogen sequence: MAGDLSAGF FMEELNTYRQ KQGVVLKYQE LPNSGPPHDR RFTFQVIIDG REFPEGEGRS KKEAKNAAAK LAVEILNKEK KAVSPLLLTT TNSSEGLSMG NYIGLINRIA QKKRLTVNYE QCASGVHGPE GFHYKCKMGQ KEYSIGTGST KQEAKQLAAK LAYLQILSEE TSVKSDYLSS GSFATTCESQ SNSLVTSTLA SESSSEGDFS ADTSEINSNS DSLNSSSLLM NGLRNNQRKA KRSLAPRFDL PDMKETKYTV DKRFGMDFKE IELIGSGGFG QVFKAKHRID GKTYVIKRVK YNNEKAEREV KALAKLDHVN IVHYNGCWDG FDYDPETSDD SLESSDYDPE NSKNSSRSKT KCLFIQMEFC DKGT (1-373 aa encoded by BC093676)

Target Information

PKR is a 68 kDa protein and an IFN-induced serine/threonine protein kinase consisting of two N-terminal RNA-binding regulatory domains and a functional C-terminal serine/threonine protein kinase domain. Activated by dsRNA produced during viral infections, PKR plays a key role in IFN induced-innate antiviral response, virus-induced apoptosis, cell growth & differentiation. Upon activation, PKR gets autophosphorylated and catalyzes the phosphorylation of elF2 alpha subunit, which leads to an inhibition of protein synthesis. PKR induces apoptosis by up-regulating Fas expression and mediates FADD/Caspase-8 death signaling pathway. Upon viral infection, it functions as a dual protein, sequentially activating both cell survival & cell death pathways using kinase independent and dependent strategies. PRKR regulates multiple pathways which include NF-kappaB activation, p53, p38, & PDGF signaling pathway. PKR has been implicated in tumor suppression & malignancy. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Defining the Role of Stress Granules in Innate Immune Suppression by the Herpes Simplex Virus 1 Endoribonuclease VHS.

Journal of virology

Burgess HM,Mohr I

Wed Aug 01 00:00:00 EDT 2018

Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis.

Nature communications

Wu H,Wei L,Fan F,Ji S,Zhang S,Geng J,Hong L,Fan X,Chen Q,Tian J,Jiang M,Sun X,Jin C,Yin ZY,Liu Q,Zhang J,Qin F,Lin KH,Yu JS,Deng X,Wang HR,Zhao B,Johnson RL,Chen L,Zhou D

Thu Feb 19 00:00:00 EST 2015

Bioinformatics

Protein Aliases: double stranded RNA activated protein kinase; dsRNA-activated kinase; EC 2.7.11.1; eIF-2 alpha; eIF-2A protein kinase 2; eIF2a Kinase; eukaryotic translation initiation factor 2 alpha kinase 2; Eukaryotic translation initiation factor 2-alpha kinase 2; IFN- type I-induced and dsRNA-activated kinase; IFN-induced and double-stranded RNA-activated kinase; Interferon-induced, double-stranded RNA-activated protein kinase; interferon-inducible elF2alpha kinase; Interferon-inducible RNA-dependent protein kinase; MGC126524; OTTHUMP00000201320; P1/eIF-2A protein kinase; p68 kinase; PKR; protein kinase R; Protein kinase RNA-activated; protein kinase, interferon inducible double stranded RNA dependent; Protein kinase, interferon-inducible double stranded RNA dependent; protein phosphatase 1, regulatory subunit 83; Serine/threonine-protein kinase TIK; T-cell viral integration site; Tyrosine-protein kinase EIF2AK2

Gene Aliases: 2310047A08Rik; 4732414G15Rik; AI467567; AI747578; EIF2AK1; EIF2AK2; PKR; PPP1R83; PRKR; Tik

UniProt ID: (Human) P19525, (Mouse) Q03963, (Rat) Q63184

Entrez Gene ID: (Human) 5610, (Mouse) 19106, (Rat) 54287

Molecular Function: non-receptor serine/threonine protein kinase