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Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:1,000-1:4,000 | View 2 publications 2 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:50-1:500 | - |
Immunocytochemistry (ICC/IF) |
1:10-1:100 | - |
Immunoprecipitation (IP) |
0.5-4.0 µg | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Rat |
Published species |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
EIF2AK2, PKR Fusion Protein Ag13010 (1-373 aa encoded by BC093676) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.21 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
This antibody is specific to EIF2AK2.
Immunogen sequence: MAGDLSAGF FMEELNTYRQ KQGVVLKYQE LPNSGPPHDR RFTFQVIIDG REFPEGEGRS KKEAKNAAAK LAVEILNKEK KAVSPLLLTT TNSSEGLSMG NYIGLINRIA QKKRLTVNYE QCASGVHGPE GFHYKCKMGQ KEYSIGTGST KQEAKQLAAK LAYLQILSEE TSVKSDYLSS GSFATTCESQ SNSLVTSTLA SESSSEGDFS ADTSEINSNS DSLNSSSLLM NGLRNNQRKA KRSLAPRFDL PDMKETKYTV DKRFGMDFKE IELIGSGGFG QVFKAKHRID GKTYVIKRVK YNNEKAEREV KALAKLDHVN IVHYNGCWDG FDYDPETSDD SLESSDYDPE NSKNSSRSKT KCLFIQMEFC DKGT (1-373 aa encoded by BC093676)
PKR is a 68 kDa protein and an IFN-induced serine/threonine protein kinase consisting of two N-terminal RNA-binding regulatory domains and a functional C-terminal serine/threonine protein kinase domain. Activated by dsRNA produced during viral infections, PKR plays a key role in IFN induced-innate antiviral response, virus-induced apoptosis, cell growth & differentiation. Upon activation, PKR gets autophosphorylated and catalyzes the phosphorylation of elF2 alpha subunit, which leads to an inhibition of protein synthesis. PKR induces apoptosis by up-regulating Fas expression and mediates FADD/Caspase-8 death signaling pathway. Upon viral infection, it functions as a dual protein, sequentially activating both cell survival & cell death pathways using kinase independent and dependent strategies. PRKR regulates multiple pathways which include NF-kappaB activation, p53, p38, & PDGF signaling pathway. PKR has been implicated in tumor suppression & malignancy. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: double stranded RNA activated protein kinase; dsRNA-activated kinase; EC 2.7.11.1; eIF-2 alpha; eIF-2A protein kinase 2; eIF2a Kinase; eukaryotic translation initiation factor 2 alpha kinase 2; Eukaryotic translation initiation factor 2-alpha kinase 2; IFN- type I-induced and dsRNA-activated kinase; IFN-induced and double-stranded RNA-activated kinase; Interferon-induced, double-stranded RNA-activated protein kinase; interferon-inducible elF2alpha kinase; Interferon-inducible RNA-dependent protein kinase; MGC126524; OTTHUMP00000201320; P1/eIF-2A protein kinase; p68 kinase; PKR; protein kinase R; Protein kinase RNA-activated; protein kinase, interferon inducible double stranded RNA dependent; Protein kinase, interferon-inducible double stranded RNA dependent; protein phosphatase 1, regulatory subunit 83; Serine/threonine-protein kinase TIK; T-cell viral integration site; Tyrosine-protein kinase EIF2AK2
Gene Aliases: 2310047A08Rik; 4732414G15Rik; AI467567; AI747578; EIF2AK1; EIF2AK2; PKR; PPP1R83; PRKR; Tik
UniProt ID: (Human) P19525, (Mouse) Q03963, (Rat) Q63184
Entrez Gene ID: (Human) 5610, (Mouse) 19106, (Rat) 54287
Molecular Function:
non-receptor serine/threonine protein kinase
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