Proteintech

EIF2B2 Polyclonal Antibody

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EIF2B2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human ovary tissue slide using 11034-1-AP ( EIF2B2 Antibody) at dilution of 1:50 (under 40x lens).

EIF2B2 Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 11034-1-AP (EIF2B2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

EIF2B2 Antibody in Immunoprecipitation (IP) — IP result of anti-EIF2B2 (IP:11034-1-AP, 4ug; Detection:11034-1-AP 1:500) with HEK-293 cells lysate 2000 ug.

Product Details

11034-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunoprecipitation (IP)	4 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EIF2B2 Fusion Protein Ag1502 (1-351 aa encoded by BC011750) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.24 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MPGSAAKGS ELSERIESFV ETLKRGGGPR SSEEMARETL GLLRQIITDH RWSNAGELME LIRREGRRMT AAQPSETTVG NMVRRVLKII REEYGRLHGR SDESDQQESL HKLLTSGGLN EDFSFHYAQL QSNIIEAINE LLVELEGTME NIAAQALEHI HSNEVIMTIG FSRTVEAFLK EAARKRKFHV IVAECAPFCQ GHEMAVNLSK AGIETTVMTD AAIFAVMSRV NKVIIGTKTI LANGALRAVT GTHTLALAAK HHSTPLIVCA PMFKLSPQFP NEEDSFHKFV APEEVLPFTE GDILEKVSVH CPVFDYVPPE LITLFISNIG GNAPSYIYRL MSELYHPDDH VL (1-351 aa encoded by BC011750)

Target Information

eIF2b beta is the beta subunit of eukaryotic initiation factor-2B (EIF2B). EIF2B is involved in protein synthesis and exchanges GDP and GTP for its activation and deactivation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

EIF2B2 mutations in vanishing white matter disease hypersuppress translation and delay recovery during the integrated stress response.

RNA (New York, N.Y.)

Moon SL,Parker R

Fri Jun 01 00:00:00 EDT 2018

Stoichiometry of the eIF2B complex is maintained by mutual stabilization of subunits.

The Biochemical journal

Wortham NC,Stewart JD,Harris S,Coldwell MJ,Proud CG

Tue Mar 01 00:00:00 EST 2016

Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response.

eLife

Sidrauski C,Tsai JC,Kampmann M,Hearn BR,Vedantham P,Jaishankar P,Sokabe M,Mendez AS,Newton BW,Tang EL,Verschueren E,Johnson JR,Krogan NJ,Fraser CS,Weissman JS,Renslo AR,Walter P

Wed Apr 15 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: eIF-2B GDP-GTP exchange factor subunit beta; eukaryotic translation initiation factor 2B, subunit 2 (beta, 39kD); eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa; S20I15; S20III15; Translation initiation factor eIF-2B subunit beta

Gene Aliases: AA409345; C85417; EIF-2Bbeta; EIF2B; EIF2B2; EIF2BB

UniProt ID: (Human) P49770, (Mouse) Q99LD9

Entrez Gene ID: (Human) 8892, (Mouse) 217715, (Rat) 84005

Molecular Function: translation initiation factor