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EIF2B4 Polyclonal Antibody

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Antibody Testing Data (11)

EIF2B4 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

EIF2B4 Antibody (11332-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed MCF-7 cells using 11332-1-AP (EIF2B4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

EIF2B4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

11332-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

EIF2B4 Fusion Protein Ag1875 (1-341 aa encoded by BC001870)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MAAVAVAVR EDSGSGMKAE LPPGPGAVGR EMTKEEKLQL RKEKKQQKKK RKEEKGAEPE TGSAVSAAQC QVGPTRELPE SGIQLGTPRE KVPAGRSKAE LRAERRAKQE AERALKQARK GEQGGPPPKA SPSTAGETPS GVKRLPEYPQ VDDLLLRRLV KKPERQQVPT RKDYGSKVSL FSHLPQYSRQ NSLTQFMSIP SSVIHPAMVR LGLQYSQGLV SGSNARCIAL LRALQQVIQD YTTPPNEELS RDLVNKLKPY MSFLTQCRPL SASMHNAIKF LNKEITSVGS SKREEEAKSE LRAAIDRYVQ EKIVLAAQAI SRFAYQKISN GDVILVYGCS SL (1-341 aa encoded by BC001870)

Target Information

Eukaryotic initiation factor 2B (EIF2B), which is necessary for protein synthesis, is a GTP exchange factor composed of five different subunits. The protein encoded by this gene is the fourth, or delta, subunit. Defects in this gene are a cause of leukoencephalopathy with vanishing white matter (VWM) and ovarioleukodystrophy. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

eIF2B conformation and assembly state regulate the integrated stress response.

eLife

Schoof M,Boone M,Wang L,Lawrence R,Frost A,Walter P

Wed Mar 10 00:00:00 EST 2021

Sugar phosphate activation of the stress sensor eIF2B.

Nature communications

Hao Q,Heo JM,Nocek BP,Hicks KG,Stoll VS,Remarcik C,Hackett S,LeBon L,Jain R,Eaton D,Rutter J,Wong YL,Sidrauski C

Tue Jun 08 00:00:00 EDT 2021

Comparative Nucleotide-Dependent Interactome Analysis Reveals Shared and Differential Properties of KRas4a and KRas4b.

ACS central science

Zhang X,Cao J,Miller SP,Jing H,Lin H

Wed Jan 24 00:00:00 EST 2018

Comparative Nucleotide-Dependent Interactome Analysis Reveals Shared and Differential Properties of KRas4a and KRas4b.

ACS central science

Zhang X,Cao J,Miller SP,Jing H,Lin H

Wed Jan 24 00:00:00 EST 2018

The small molecule ISRIB rescues the stability and activity of Vanishing White Matter Disease eIF2B mutant complexes.

eLife

Wong YL,LeBon L,Edalji R,Lim HB,Sun C,Sidrauski C

Wed Feb 28 00:00:00 EST 2018

Stoichiometry of the eIF2B complex is maintained by mutual stabilization of subunits.

The Biochemical journal

Wortham NC,Stewart JD,Harris S,Coldwell MJ,Proud CG

Tue Mar 01 00:00:00 EST 2016

Stoichiometry of the eIF2B complex is maintained by mutual stabilization of subunits.

The Biochemical journal

Wortham NC,Stewart JD,Harris S,Coldwell MJ,Proud CG

Tue Mar 01 00:00:00 EST 2016

Paradoxical Sensitivity to an Integrated Stress Response Blocking Mutation in Vanishing White Matter Cells.

PloS one

Sekine Y,Zyryanova A,Crespillo-Casado A,Amin-Wetzel N,Harding HP,Ron D

Wed Jun 28 00:00:00 EDT 2017

Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response.

eLife

Sidrauski C,Tsai JC,Kampmann M,Hearn BR,Vedantham P,Jaishankar P,Sokabe M,Mendez AS,Newton BW,Tang EL,Verschueren E,Johnson JR,Krogan NJ,Fraser CS,Weissman JS,Renslo AR,Walter P

Wed Apr 15 00:00:00 EDT 2015

Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response.

eLife

Sidrauski C,Tsai JC,Kampmann M,Hearn BR,Vedantham P,Jaishankar P,Sokabe M,Mendez AS,Newton BW,Tang EL,Verschueren E,Johnson JR,Krogan NJ,Fraser CS,Weissman JS,Renslo AR,Walter P

Wed Apr 15 00:00:00 EDT 2015

Analysis of the subunit organization of the eIF2B complex reveals new insights into its structure and regulation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Wortham NC,Martinez M,Gordiyenko Y,Robinson CV,Proud CG

Thu May 01 00:00:00 EDT 2014

Analysis of the subunit organization of the eIF2B complex reveals new insights into its structure and regulation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Wortham NC,Martinez M,Gordiyenko Y,Robinson CV,Proud CG

Thu May 01 00:00:00 EDT 2014

Analysis of the subunit organization of the eIF2B complex reveals new insights into its structure and regulation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Wortham NC,Martinez M,Gordiyenko Y,Robinson CV,Proud CG

Thu May 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: DKFZp586J0119; eIF-2B GDP-GTP exchange factor subunit delta; eIF2B GDP-GTP exchange factor subunit delta; eukaryotic translation initiation factor 2B subunit 4 delta; eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa; translation initiation factor eIF-2b delta subunit; translation initiation factor eIF-2B subunit delta; Translation initiation factor eIF2B subunit delta

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Gene Aliases: EIF-2B; EIF2B; EIF2B4; EIF2BD; EIF2Bdelta; Jgr1a

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UniProt ID: (Human) Q9UI10, (Rat) Q63186, (Mouse) Q61749

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Entrez Gene ID: (Human) 8890, (Rat) 117019, (Mouse) 13667

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Function(s)

translation initiation factor activity guanyl-nucleotide exchange factor activity protein binding translation initiation factor binding S-methyl-5-thioribose-1-phosphate isomerase activity translation initiation factor

Process(es)

ovarian follicle development translational initiation regulation of translation response to heat response to glucose oligodendrocyte development L-methionine biosynthetic process from methylthioadenosine negative regulation of translational initiation in response to stress myelination response to peptide hormone positive regulation of GTPase activity negative regulation of translational initiation cellular response to stimulus cellular metabolic process translation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EIF2B4 Polyclonal Antibody

EIF2B4 Antibody (11332-1-AP) in ICC/IF

Product Details

11332-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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