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Invitrogen
EIF2S1 Polyclonal Antibody
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FIGURE: 1 / 9
EIF2S1 Antibody (PA5-27366) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of eIF2a was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: eIF2a Polyclonal Antibody (Product # PA5-27366) diluted at 1:5000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-27366
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans,
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human eIF2 alpha. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.63 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: A549, H1299, HCT-116, rat neuron, HeLa, N2a.
Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (92%), Chicken (97%), Rhesus Monkey (100%), Bovine (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
EIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner to form the 43S pre-initiation complex. The polypeptide can be phosphorylated by related protein kinases activated in response to stress. Phosphorylated EIF2A inhibits EIF2B activity and prevents guanine nucleotide exchange.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: eIF-2-alpha; EIF-2A; EIF-2alpha; eIF2 alpha; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; eukaryotic translation initiation factor 2A
Gene Aliases: 0910001O23Rik; 2410026C18Rik; 35kDa; EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A; eIF2alpha; EIF2S1
UniProt ID: (Human) P05198, (Rat) P68101, (Mouse) Q6ZWX6
Entrez Gene ID: (Human) 1965, (Rat) 54318, (Mouse) 13665
positive regulation of defense response to virus by host
translational initiation
aging
negative regulation of translational initiation in response to stress
cellular response to amino acid starvation
cellular response to heat
cellular response to UV
response to endoplasmic reticulum stress
PERK-mediated unfolded protein response
protein autophosphorylation
transmembrane transport
mitophagy in response to mitochondrial depolarization
xenophagy
positive regulation of neuron death
response to manganese-induced endoplasmic reticulum stress
positive regulation of type B pancreatic cell apoptotic process
translation
regulation of translational initiation in response to stress
regulation of translation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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