|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 55 and 328 of Human EIF3F|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 40% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-27585 targets EIF3F in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-27585 immunogen is recombinant fragment corresponding to a region within amino acids 55 and 328 of Human EIF3F.
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 47kD); 47kDa; Deubiquitinating enzyme eIF3f; eIF-3-epsilon; eIF3 p47; eIF3-epsilon; eIF3-p47; EIF3F; EIF3S5; eukaryotic translation initiation factor 3; Eukaryotic translation initiation factor 3 subunit 5; Eukaryotic translation initiation factor 3 subunit F; eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47kD); eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa; eukaryotic translation initiation factor 3, subunit F; subunit 5 (epsilon; subunit 5 epsilon
Gene Aliases: eIF3-p47; EIF3F; EIF3S5
UniProt ID: (Human) O00303
Entrez Gene ID: (Human) 8665
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