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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500-1:1,000 | View 1 publication 1 publication |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:20-1:200 | - |
Immunocytochemistry (ICC/IF) |
1:20-1:200 | View 1 publication 1 publication |
Immunoprecipitation (IP) |
0.5-4.0 µg | View 1 publication 1 publication |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Rat |
Published species |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
EIF3J Fusion Protein Ag0678 (1-258 aa encoded by BC002719) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.22 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.1% sodium azide |
Storage conditions |
-20°C |
Immunogen sequence: MAAAAAAAG DSDSWDADAF SVEDPVRKVG GGGTAGGDRW EGEDEDEDVK DNWDDDDDEK KEEAEVKPEV KISEKKKIAE KIKEKERQQK KRQEEIKKRL EEPEEPKVLT PEEQLADKLR LKKLQEESDL ELAKETFGVN NAVYGIDAMN PSSRDDFTEF GKLLKDKITQ YEKSLYYASF LEVLVRDVCI SLEIDDLKKI TNSLTVLCSE KQKQEKQSKA KKKKKGVVPG GGLKATMKDD LADYGGYDGG YVQDYEDFM (1-258 aa encoded by BC002719 )
Eukaryotic initiation factor-3 (EIF3) has a molecular mass of about 600 kD and contains 13 nonidentical protein subunits, including EIF3J. EIF3 plays a central role in binding of initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to form the 40S initiation complex (Fraser et al., 2004; Fraser et al., 2007).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: eIF-3-alpha; eIF-3-alpha {ECO:0000255|HAMAP-Rule:MF_03009}; eIF-3-alpha-A; eIF-3-alpha-B; eIF3 p35; eIF3 p35 {ECO:0000255|HAMAP-Rule:MF_03009}; EIF3J; eIF3j {ECO:0000255|HAMAP-Rule:MF_03009}; eIF3j-A; eIF3j-B; Eukaryotic translation initiation factor 3 subunit 1; eukaryotic translation initiation factor 3 subunit 1 {ECO:0000255|HAMAP-Rule:MF_03009}; Eukaryotic translation initiation factor 3 subunit 1-A; Eukaryotic translation initiation factor 3 subunit 1-B; Eukaryotic translation initiation factor 3 subunit J; eukaryotic translation initiation factor 3 subunit J {ECO:0000255|HAMAP-Rule:MF_03009}; Eukaryotic translation initiation factor 3 subunit J-A; Eukaryotic translation initiation factor 3 subunit J-B; eukaryotic translation initiation factor 3, subunit 1 (alpha, 35kD); eukaryotic translation initiation factor 3, subunit 1 alpha; eukaryotic translation initiation factor 3, subunit J
Gene Aliases: 1810016I04Rik; 2700079K05Rik; AA409117; AA409446; C78575; eIF-3-alpha-A; eIF-3-alpha-B; eIF3 p35; eIF3-alpha; eIF3-p35; EIF3J; Eif3j-1; Eif3j-2; eIF3j-A; eIF3j-B; Eif3j1; Eif3j2; EIF3S1; Eif3s1-1; Eif3s1-2; ENSMUSG00000043424; Gm9781; PRO0391
UniProt ID: (Human) O75822, (Rat) A0JPM9, (Mouse) Q3UGC7, (Mouse) Q66JS6
Entrez Gene ID: (Human) 8669, (Rat) 691947, (Mouse) 78655, (Mouse) 100042807
Molecular Function:
translation initiation factor
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