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EIF4G1 Polyclonal Antibody

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EIF4G1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using 15704-1-AP (EIF4G1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

EIF4G1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 15704-1-AP (EIF4G1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

EIF4G1 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 15704-1-AP (EIF4G1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

EIF4G1 Antibody in Immunoprecipitation (IP) — IP result of anti-EIF4G1 (IP:15704-1-AP, 5ug; Detection:15704-1-AP 1:500) with HeLa cells lysate 2000ug.

Product Details

15704-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EIF4G1 Fusion Protein Ag8342 (296-645 aa encoded by BC007788) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.23 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: IEEYLHLND MKEAVQCVQE LASPSLLFIF VRHGVESTLE RSAIAREHMG QLLHQLLCAG HLSTAQYYQG LYEILELAED MEIDIPHVWL YLAELVTPIL QEGGVPMGEL FREITKPLRP LGKAASLLLE ILGLLCKSMG PKKVGTLWRE AGLSWKEFLP EGQDIGAFVA EQKVEYTLGE ESEAPGQRAL PSEELNRQLE KLLKEGSSNQ RVFDWIEANL SEQQIVSNTL VRALMTAVCY SAIIFETPLR VDVAVLKARA KLLQKYLCDE QKELQALYAL QALVVTLEQP PNLLRMFFDA LYDEDVVKED AFYSWESSKD PAEQQGKGVA LKSVTAFFKW LREAEEESDH N (296-645 aa encoded by BC007788)

Target Information

The protein encoded by this gene is a component of the protein complex EIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure, and recruitment of mRNA to the ribosome. Alternative splicing results in five transcript variants encoding four distinct isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cellular cap-binding protein, eIF4E, promotes picornavirus genome restructuring and translation.

Proceedings of the National Academy of Sciences of the United States of America

Avanzino BC,Fuchs G,Fraser CS

Tue Sep 05 00:00:00 EDT 2017

Construction of a subgenomic CV-B3 replicon expressing emerald green fluorescent protein to assess viral replication of a cardiotropic enterovirus strain in cultured human cells.

Journal of virological methods

Wehbe M,Huguenin A,Leveque N,Semler BL,Hamze M,Andreoletti L,Bouin A

Fri Apr 01 00:00:00 EDT 2016

SAMHD1 Inhibits LINE-1 Retrotransposition by Promoting Stress Granule Formation.

PLoS genetics

Hu S,Li J,Xu F,Mei S,Le Duff Y,Yin L,Pang X,Cen S,Jin Q,Liang C,Guo F

Wed Jul 01 00:00:00 EDT 2015

Protease 2A induces stress granule formation during coxsackievirus B3 and enterovirus 71 infections.

Virology journal

Wu S,Wang Y,Lin L,Si X,Wang T,Zhong X,Tong L,Luan Y,Chen Y,Li X,Zhang F,Zhao W,Zhong Z

Thu Nov 20 00:00:00 EST 2014

Bioinformatics

Protein Aliases: DKFZp686A1451; eIF-4-gamma 1; EIF4-gamma; eucaryotic translation initiation factor 4G; Eukaryotic translation initiation factor 4 gamma 1; eukaryotic translation initiation factor 4 gamma, 1; OTTHUMP00000210577; OTTHUMP00000210586; OTTHUMP00000210587; OTTHUMP00000210588; OTTHUMP00000210595; OTTHUMP00000210602; OTTHUMP00000210603; p220

Gene Aliases: EIF-4G1; EIF4F; EIF4G; EIF4G1; EIF4GI; P220; PARK18

UniProt ID: (Human) Q04637

Entrez Gene ID: (Human) 1981

Molecular Function: translation factor translation initiation factor translational protein

Function(s)

nuclear-transcribed mRNA catabolic process, nonsense-mediated decay nuclear-transcribed mRNA poly(A) tail shortening behavioral fear response translational initiation regulation of translational initiation viral process regulation of mRNA stability positive regulation of neuron differentiation cell-cell adhesion

Process(es)

translation initiation factor activity protein binding translation factor activity, RNA binding eukaryotic initiation factor 4E binding poly(A) RNA binding cadherin binding involved in cell-cell adhesion

EIF4G1 Polyclonal Antibody

Product Details

15704-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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