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Agrisera
EIN2/Ethylene insensitive 2 Polyclonal Antibody
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FIGURE: 1 / 1
EIN2/Ethylene insensitive 2 Antibody (AS12-1865) in WB
40-60 µg of membrane protein (measured with Millipore Direct Detect spec. ) isolated following membrane extraction buffer protocol from Dong et al. 2008 (Plant Journal 53(2): 275-286) from 4 day old dark grown Arabidopsis thaliana seedlings, grown on MS plated with ACC or AVG in the plate, extracted with (10 m M Tris-HCl p H 7.5,150 m M Na Cl, 1 m M EDTA, 10% glycerol, 1% Triton X-100, and protease inhibito... View More
Product Details
AS12-1865
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated peptide chosen from EIN2 of Arabidopsis thaliana.
Conjugate
Form
Lyophilized
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
Central factor in signaling pathways regulated by ethylene (ET) and involved in various processes including development, plant defense, senescence, nucleotide sugar flux, and tropisms (PubMed:27694846, PubMed:28874528). Necessary for ethylene-mediated gene regulation, and for the induction of some genes by ozone. Acts downstream of ET receptors, and upstream of ethylene regulated transcription factors. Required for cytokinin-mediated processes. Seems to be implicated in cross-talk between ET, jasmonate and other pathways. Probably not involved in iron uptake (PubMed:10381874, PubMed:12953109, PubMed:14973160, PubMed:15010611, PubMed:15272873, PubMed:7770519, PubMed:9351240). Has a short half-life and undergoes rapid proteasome-mediated turnover in the absence of ethylene (PubMed:19196655). Required for ethylene-induced EIN3 stabilization via proteasomal degradation of EBF1/EBF2 proteins (PubMed:20647342). Regulates the leaf senescence induced by methyl jasmonate, ethylene and abscisic acid (Ref.17, PubMed:15010611). Required during salt stress to confer resistance (PubMed:21631530).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATEIN2; CKR1; CYTOKININ RESISTANT 1; Cytokinin-resistant protein AtCKR1; ENHANCED RESPONSE TO ABA3; ERA3; ETHYLENE INSENSITIVE 2; Ethylene-insensitive protein 2; F12E4.10; F12E4_10; ORE2; ORE3; ORESARA 2; ORESARA 3; PIR2; Protein ORESARA 3
Gene Aliases: AT5G03280; ATEIN2; CKR1; CYTOKININ RESISTANT 1; EIN2; ENHANCED RESPONSE TO ABA3; ERA3; ETHYLENE INSENSITIVE 2; F12E4.10; F12E4_10; MOK16.19; ORE2; ORE3; ORESARA 2; ORESARA 3; PIR2
UniProt ID: (Arabidopsis) Q9S814
Entrez Gene ID: (Arabidopsis) 831889
establishment of planar polarity
response to molecule of bacterial origin
response to osmotic stress
response to oxidative stress
cell death
response to heat
response to salt stress
response to ethylene
response to hormone
response to jasmonic acid
positive regulation of abscisic acid-activated signaling pathway
jasmonic acid and ethylene-dependent systemic resistance, ethylene mediated signaling pathway
ethylene-activated signaling pathway
auxin polar transport
phloem or xylem histogenesis
regulation of stomatal movement
leaf senescence
sugar mediated signaling pathway
negative regulation of defense response
defense response to bacterium
root hair cell differentiation
defense response to fungus
cell division
defense response by callose deposition in cell wall
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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