|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptides corresponding to residues M(1) A A T C E I S N I F S N Y F S(16) and S(335) S G W K E E E V L Q S R N(348) of human ESE-1.|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500 - 1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-4221 detects the ESE-1 protein in human samples.
PA1-4221 has been successfully used in Western blot procedures. By Western blot this antibody detects both isoforms of ESE-1 protein, both bands around ~40 kDa, in T84 colon carcinoma cells.
The PA1-4221 immunogen is a synthetic peptides corresponding to residues M(1) A A T C E I S N I F S N Y F S(16) and S(335) S G W K E E E V L Q S R N(348) of the N- and C-termini of human ESE-1.
ESE-1 (for epithelium-specific Ets), is a novel, highly tissue-restricted member of the ets transcription factor/oncogene family, which has features distinct from those of any other ets-related factor. ESE-1 binds with high affinity to and transactivates the ets binding site in the promoter of the keratinocyte terminal differentiation marker gene, SPRR2A. ESE-1 is a critical regulator of epithelial cell differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.