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Invitrogen
ELF3 Polyclonal Antibody
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FIGURE: 1 / 2
ELF3 Antibody (PA5-89261) in WB
Western blot analysis of ELF3 in various lysates. Samples were incubated with ELF3 Polyclonal antibody (Product # PA5-89261) using a dilution of 1:400, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
Product Details
PA5-89261
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ESE1/ESE1/ELF3 (NP_0044243)
Conjugate
Form
Liquid
Concentration
0.48 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MAATCEISNI FSNYFSAMYS SEDSTLASVP PAATFGADDL VLTLSNPQMS LEGTEKASWL GEQPQFWSKT QVLDWISYQV EKNKYDASAI DFSRCDMDGA TLCNCALEEL RLVFGPLGDQ LHAQLRDLTS SSSDELSWII ELLEKDGMAF QEALDPGPFD QGSPFAQELL DDGQQASPYH PGSCGAGAPS PGSSDVSTAG TGASRSSHSS DSGGSDVDLD PTDGKLFPSD GFRDCKKGDP KHGKRKRGRP RKLSKEYWDC LEGKKSKHAP RGTHLWEFIR; Positive Samples: Mouse skin, Mouse lung, SGC-7901, HT-29, SW480; Cellular Location: Cytoplasm, Nucleus
Target Information
ESE-1 (Epithelium-Specific Ets) is a novel, highly tissue-restricted member of the ets transcription factor/oncogene family, which has features distinct from those of any other ets-related factor. ESE-1 binds with high affinity to and transactivates the ets binding site in the promoter of the keratinocyte terminal differentiation marker gene, SPRR2A. ESE-1 is a critical regulator of epithelial cell differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: E74-like factor 3; E74-like factor 3 (ets domain transcription factor); E74-like factor 3 (ets domain transcription factor, epithelial-specific ); E74-like factor 3 (ETS domain transcription factor, serine box, epithelial-specific); ELF3; Epithelial-restricted with serine box; Epithelium-restricted Ets protein ESX; Epithelium-specific Ets transcription factor 1; ESE-1; ets domain transcription factor, serine box (epithelial-specific); ETS-related transcription factor Elf-3; RP11-510N19.1
Gene Aliases: ELF3; EPR-1; ERT; ESE-1; ESX; JEN
UniProt ID: (Human) P78545, (Mouse) Q3UPW2
Entrez Gene ID: (Human) 1999, (Mouse) 13710
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
transcription coactivator activity
protein binding
DNA binding
sequence-specific DNA binding
winged helix/forkhead transcription factor
blastocyst development
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
inflammatory response
multicellular organism development
epidermis development
extracellular matrix organization
epithelial cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
mammary gland involution
transcription, DNA-templated
regulation of transcription, DNA-templated
anatomical structure morphogenesis
embryo development
cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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