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Invitrogen
ELF5 Polyclonal Antibody
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FIGURE: 1 / 4
ELF5 Antibody (720380) in ICC/IF
For immunofluorescence analysis, Ntera2 cells were fixed and permeabilized for detection of endogenous ELF5 using Anti- ELF5 Rabbit polyclonal Antibody (Product # 720380, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of ELF5 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c)... View More
Product Details
720380
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptides corresponding to human ELF5 [1) aa131-aa149, 2) aa252-aa265]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 30% glycerol, 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Rat, Rabbit
Target Information
ELF5 is a member of an epithelium-specific subclass of the Ets transcritpion factor family. In addition to its role in regulating the later stages of terminal differentiation of keratinocytes, it appears to regulate a number of epithelium-specific genes found in tissues containing glandular epithelium such as salivary gland and prostate. It has very low affinity to DNA due to its negative regulatory domain at the amino terminus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: E74-like factor 5; E74-like factor 5 (ets domain transcription factor); elf-5; Epithelium specific Ets-2; Epithelium-restricted ESE-1-related Ets factor; Epithelium-specific Ets transcription factor 2; ESE-2; ETS-related transcription factor Elf-5
Gene Aliases: ELF5; ESE-2; ESE-5; ESE-5.; ESE2
UniProt ID: (Human) Q9UKW6, (Mouse) Q8VDK3
Entrez Gene ID: (Human) 2001, (Mouse) 13711, (Rat) 366142
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
winged helix/forkhead transcription factor
ectodermal cell fate commitment
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
cell proliferation
cell differentiation
somatic stem cell population maintenance
negative regulation of cell differentiation
positive regulation of transcription from RNA polymerase II promoter
mammary gland epithelial cell differentiation
transcription, DNA-templated
regulation of transcription, DNA-templated
ectoderm development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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