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EMAP II Antibody (500-P172G-100UG) in WB
Western Blot: To detect Human EMAP-II by Western Blot analysis EMAP II Polyclonal Antibody (Product # 500-P172G-1MG) can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human EMAP-II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P172G-100UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human EMAP-II
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929623
Product Specific Information
AA Sequence of recombinant protein: discontinued.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human EMAP-II. Anti-Human EMAP-II-specific antibody was purified by affinity chromatography employing an immobilized Human EMAP-II matrix.
Sandwich ELISA: To detect Human EMAP-II by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human EMAP-II (500-P172GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human EMAP-II.
Western Blot: To detect Human EMAP-II by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human EMAP-II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Aminoacyl tRNA synthase complex-interacting multifunctional protein 1; aminoacyl tRNA synthetase complex-interacting multifunctional protein 1; ARS-interacting multifunctional protein 1; endothelial monocyte-activating polypeptide 2; endothelial-monocyte activating polypeptide II; mca1; Multisynthase complex auxiliary component p43; multisynthetase complex auxiliary component p43; small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating)
Gene Aliases: AIMP1; EMAP2; EMAPII; HLD3; p43; SCYE1
UniProt ID: (Human) Q12904
Entrez Gene ID: (Human) 9255
angiogenesis
negative regulation of endothelial cell proliferation
glucose metabolic process
tRNA aminoacylation for protein translation
apoptotic process
chemotaxis
inflammatory response
cell adhesion
signal transduction
cell-cell signaling
response to wounding
leukocyte migration
defense response to virus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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