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          • Primary Antibodies ›
          • EMP Antibodies

          Invitrogen

          EMP Polyclonal Antibody

          View all (11) EMP antibodies

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          Cite EMP Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          EMP Antibody in Immunocytochemistry (ICC/IF)
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          EMP Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          EMP Antibody (PA5-66147) in ICC/IF

          Immunofluorescent staining of EMP in human cell line U-251 MG shows localization to nucleoplasm. Samples were probed using an EMP Polyclonal Antibody (Product # PA5-66147). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          EMP Antibody in Immunocytochemistry (ICC/IF)
          EMP Antibody in Immunohistochemistry (IHC)
          EMP Polyclonal Antibody

          Product Details

          PA5-66147

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:500-1:1,000
          -

          Immunocytochemistry (ICC/IF)

          0.25-2 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant Human EMP. Recombinant protein control fragment (Product #RP-106828).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.4 mg/mL

          Amount

          40 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.2, with 40% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2663820

          Product Specific Information

          Immunogen sequence: GVVEKLSVLKR KAVESIQAED ESAKLCKRRI EHLKEHSSDQ PAAASVWKRK RMDRMMVEHL LRCGYYNTAV KL

          Highest antigen sequence identity to the following orthologs - mouse 99%, rat 99%.

          Target Information

          This gene product mediates the attachment of erythroblasts to macrophages. This attachment promotes terminal maturation and enucleation of erythroblasts, presumably by suppressing apoptosis. This protein is an integral membrane protein with the N-terminus on the extracellular side and the C-terminus on the cytoplasmic side of the cell. Two immunologically related isoforms of erythroblast macrophage protein with apparent molecular weights of 33 kD and 36 kD were detected in macrophage membranes, this gene encodes the larger isoform. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Cell proliferation-inducing gene 5 protein; E3 ubiquitin-protein transferase MAEA; Erythroblast macrophage protein; GID complex subunit 9, FYV10 homolog; HLC-10; Human lung cancer oncogene 10 protein; lung cancer-related protein 10; Macrophage erythroblast attacher; P44EMLP; PIG5; unnamed protein product

          View more View less

          Gene Aliases: EMLP; EMP; GID9; HLC-10; HLC10; MAEA; P44EMLP; PIG5

          View more View less

          UniProt ID: (Human) Q7L5Y9

          View more View less

          Entrez Gene ID: (Human) 10296

          View more View less

          Function(s)
          actin binding ubiquitin-protein transferase activity zinc ion binding transferase activity metal ion binding ubiquitin protein ligase activity
          Process(es)
          cytoskeleton organization cell adhesion regulation of mitotic cell cycle negative regulation of myeloid cell apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process erythrocyte maturation erythrocyte development enucleate erythrocyte development cell division
          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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