Invitrogen

EMP2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (3) EMP2 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

EMP2 Antibody in Immunofluorescence (IF) — Immunofluorescent staining of EMP2 in human cell line U-251 MG using an EMP2 Polyclonal Antibody (Product # PA5-53220) shows localization to nucleoplasm.

EMP2 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of EMP2 in human pancreas using EMP2 Polyclonal Antibody (Product # PA5-53220) shows moderate to strong cytoplasmic positivity in exocrine glandular cells.

EMP2 Antibody in Western Blot (WB) — Western blot analysis of EMP2 in control (vector only transfected HEK293T lysate) and EMP2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a EMP2 Polyclonal Antibody (Product # PA5-53220).

EMP2 Antibody in Relative expression — Relative expression in different tissues in IHC: Detection of differential expression levels of EMP2 demonstrates antibody specificity. Immunohistochemical analysis of EMP2 using anti-EMP2 Polyclonal Antibody (Product # PA5-53220), shows significant staining of EMP2 in human lung and shows minimal or weak staining in human pancreas tissues. The relative expression levels of EMP2 within each tissue is shown using RNA-Seq. {RE}

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

0.25-2 µg/mL

Immunofluorescence (IF)

0.25-2 µg/mL

Immunohistochemistry (IHC)

1:50-1:200

Western Blot (WB)

0.04-0.4 µg/mL

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human EMP2

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2641027

Product Specific Information

Immunogen sequence: DNAWWVGDEF FADVWRICTN NTNCTVINDS FQEYST

Highest antigen sequence identity to the following orthologs: Mouse - 61%, Rat - 68%.

Target Information

Epithelial membrane protein-2 (EMP2) is a member of the four transmembrane superfamily (TM4SF) and is thought to mediate trafficking of diverse proteins such as alpha6beta1 integrin and MHC class I to lipid raft microdomains. EMP2 has also recently been recognized as a putative tumor suppressor gene in certain model systems.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.