Invitrogen

EMP2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (4) EMP2 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

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EMP2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of EMP2 in human cell line U-251 MG using an EMP2 Polyclonal Antibody (Product # PA5-53220) shows localization to nucleoplasm.

EMP2 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of EMP2 in human pancreas using EMP2 Polyclonal Antibody (Product # PA5-53220) shows moderate to strong cytoplasmic positivity in exocrine glandular cells.

EMP2 Antibody in Western Blot (WB) — Western blot analysis of EMP2 in control (vector only transfected HEK293T lysate) and EMP2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a EMP2 Polyclonal Antibody (Product # PA5-53220).

EMP2 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of EMP2 demonstrates antibody specificity. Immunohistochemical analysis of EMP2 using anti-EMP2 Polyclonal Antibody (Product # PA5-53220), shows significant staining of EMP2 in human lung and shows minimal or weak staining in human pancreas tissues. The relative expression levels of EMP2 within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-53220

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (IHC)	1:50-1:200	-
Immunocytochemistry (ICC/IF)	0.25-2 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human EMP2 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2641027

Product Specific Information

Immunogen sequence: DNAWWVGDEF FADVWRICTN NTNCTVINDS FQEYST

Highest antigen sequence identity to the following orthologs: Mouse - 61%, Rat - 68%.

Target Information

Epithelial membrane protein-2 (EMP2) is a member of the four transmembrane superfamily (TM4SF) and is thought to mediate trafficking of diverse proteins such as alpha6beta1 integrin and MHC class I to lipid raft microdomains. EMP2 has also recently been recognized as a putative tumor suppressor gene in certain model systems.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.