Invitrogen

EMR1 Polyclonal Antibody

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EMR1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using EMR1 (Product # PA5-21399) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

EMR1 Antibody in Western Blot (WB) — Western Blot using EMR1 Polyclonal Antibody (Product # PA5-21399). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EMR1 Polyclonal Antibody (Product # PA5-21399) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Product Details

PA5-21399

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 626 and 886 of EMR1 (Uniprot ID#Q14246) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.23 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol, 1% BSA
Contains	0.025% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_11154537

Product Specific Information

Recommended positive controls: Jurkat, Raji, K562, C8D30, NIH-3T3, Raw264.7, C2C12, rat liver.

Predicted reactivity: Mouse (86%), Rat (84%), Dog (84%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

EMR1 has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. EMR1 is the human ortholog of murine F4/80 but unlike F4/80, the expression of EMR1 in humans is restricted to eosinophils and is not expressed on macrophage populations.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cardioprotective actions of relaxin.

Molecular and cellular endocrinology

Martin B,Romero G,Salama G

PA5-21399 was used in Immunocytochemistry-immunoflourescence to investigate the mechanisms underlying the hormone relaxin''s role in pregnancy.

Wed May 01 00:00:00 EDT 2019

Relaxin reverses inflammatory and immune signals in aged hearts.

PloS one

Martin B,Gabris-Weber BA,Reddy R,Romero G,Chattopadhyay A,Salama G

PA5-21399 was used in Immunohistochemistry-immunofluorescence to support the hypothesis that relaxin alters gene transcription and suppresses inflammatory pathways and genes associated with HF and aging.

Wed Feb 21 00:00:00 EST 2018

Bioinformatics

Protein Aliases: Adhesion G protein-coupled receptor E1; Adhesion G protein-coupled receptor E2; cell surface glycoprotein EMR1; Cell surface glycoprotein F4/80; egf-like module containing, mucin-like, hormone receptor-like 1; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; lymphocyte antigen 71

Gene Aliases: ADGRE1; DD7A5-7; EGF-TM7; EMR1; F4/80; Gpf480; Ly71; TM7LN3

UniProt ID: (Human) Q14246, (Mouse) Q61549, (Rat) Q5Y4N8

Entrez Gene ID: (Human) 2015, (Mouse) 13733, (Rat) 316137

Molecular Function: G-protein coupled receptor