Invitrogen

EMX1 Polyclonal Antibody

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EMX1 Antibody in Western Blot (WB) — Western blot analysis of EMX1 in U251 and Neuro-2a cell line mouse cerebellum tissue lysates (35 µg/lane) using an EMX1 polyclonal antibody (Product # PA5-35373).

EMX1 Antibody in Immunohistochemistry (IHC) — Fig. 1 4EHP expression in the brain. a - c Developmental expression of 4EHP, GIGYF2, and GAPDH in the cortex, hippocampus, and cerebellum, respectively, as measured by western blot. Quantification of a, b, and c (lower panel, n = 3 per group, normalized to the average of all age points for each protein per membrane). d 4EHP expression in a synaptosome preparation (left panel). PSD95 was enriched in the synaptosome (Syn) as opposed to the cytosol (Cyto), demonstrating proper synaptosome preparation. GAPDH and beta-actin were used as loading controls. Quantification of d (right panel, n = 3). e Primary neurons were derived from the hippocampus of wild-type mice and cultured for 14 d. Immunofluorescent analysis confirmed 4EHP expression in the synapse by colocalization with PSD95 (merge). The scale bar represents 20 um in the upper panel of images and 5 um in the lower panel of images. The lower panel of images correspond to 4 x zoom of the upper panel of images defined by the white box. Analysis of cell-type-specific expression of 4EHP by colocalization with f Empty Spiracles Homeobox 1 (EMX1, defining excitatory neurons), g parvalbumin (PV, defining a subset of inhibitory neurons), h somatostatin (SST, defining another subset of inhibitory neurons), and I laminin (LAMA1, defining endothelial cells) in the hippocampus of wild-type mice. 4EHP expression is colored in red, the cell type marker in green, and Hoechst-stained nucleus in blue. Arrows indicate a positive signal for th

Product Details

PA5-35373

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

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Immunohistochemistry (IHC)

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Immunohistochemistry (PFA fixed) (IHC (PFA))

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Immunocytochemistry (ICC/IF)

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Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 228-257 amino acids from the C-terminal region of human EMX1

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

2.0 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2552683

Target Information

EMX1 is a transcription factor, which in cooperation with EMX2. It acts to generate the boundary between the roof and archipallium in the developing brain. It may function in combinations with OTX1/2 to specify cell fates in the developing central nervous system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The eIF4E homolog 4EHP (eIF4E2) regulates hippocampal long-term depression and impacts social behavior.

Molecular autism

Wiebe S,Meng XQ,Kim SH,Zhang X,Lacaille JC,Aguilar-Valles A,Sonenberg N

PA5-35373 was used in Immunohistochemistry (PFA fixed) to investigate the effect of GRB10 interacting GYF protein 2(GIGYF2) mutations on eIF4E homologous protein(EHP) function and its association with autism spectrum disorder (ASD).

Mon Nov 23 00:00:00 EST 2020

The eIF4E homolog 4EHP (eIF4E2) regulates hippocampal long-term depression and impacts social behavior.

Molecular autism

Wiebe S,Meng XQ,Kim SH,Zhang X,Lacaille JC,Aguilar-Valles A,Sonenberg N

Mon Nov 23 00:00:00 EST 2020

16p11.2 microdeletion imparts transcriptional alterations in human iPSC-derived models of early neural development.

eLife

Roth JG,Muench KL,Asokan A,Mallett VM,Gai H,Verma Y,Weber S,Charlton C,Fowler JL,Loh KM,Dolmetsch RE,Palmer TD

PA5-35373 was used in Immunocytochemistry-immunoflourescence to reveal disruptions in gene programs related to both nervous system function and cellular metabolism.