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ENC-2 Antibody (PA5-31115) in IHC (P)
ENC-2 Polyclonal Antibody detects KLHL25 protein at cytosol on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. ENC-2 Polyclonal Antibody (Product # PA5-31115) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Product Details
PA5-31115
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 360 and 589 of Human KLHL25
Conjugate
Form
Liquid
Concentration
0.69 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2548589
Product Specific Information
Recommended positive controls: NT2D1.
Predicted reactivity: Mouse (96%), Rat (95%), Xenopus laevis (92%), Rhesus Monkey (99%), Bovine (97%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
KLHL25 (ectoderm-neural cortex protein 2, ENC2) is a cytoplasmic protein that contains six Kelch regions and a single BTB (POZ) domain. KLHL25 is highly homologus to another Kelch-like protein, ENC1, and it is believed to operate in a manner similar to other Kelch-domain containing proteins. Kelch-domain repeat containing proteins often act as modifiers of Actin fibers. Expressed early in embryogenesis, ENC1 helps to mediate neuronal process formation. It also appears to have a role in neural crest cell differentiation. KLHL25 likely functions as a substrate specific adapter for protein ubiquitinating complexes. KLHL25 is expressed in most tissues with highest expression in brain and liver.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BTB/POZ KELCH domain protein; Ectoderm-neural cortex protein 2; ectoderm-neural cortex-1; ectodermal-neural cortex 2; ENC-2; FLJ12587; FLJ45015; kelch-like 25; kelch-like family member 25; Kelch-like protein 25
Gene Aliases: 2810402K13Rik; BC027373; Enc-1; ENC-2; ENC2; KLHL25; RGD1310815
UniProt ID: (Human) Q9H0H3, (Rat) Q4KLM4, (Mouse) Q8R2P1
Entrez Gene ID: (Human) 64410, (Rat) 293023, (Mouse) 207952
Performance Guarantee
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