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Invitrogen
ENDOG Polyclonal Antibody
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FIGURE: 1 / 6
ENDOG Antibody (PA5-20004) in IHC (P)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using ENDOG Polyclonal Antibody (Product # PA5-20004) at 2.5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as seconda... View More
Product Details
PA5-20004
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to 15 amino acids near the amino terminus of human EndoG.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In Western blot applications, this antibody detects a band at ~35kDa. A suggested positive control is HepG2 cell lysate.
PA5-20004 can be used with blocking peptide PEP-0126.
Target Information
The protein encoded by this gene is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein is widely distributed among animals and cleaves DNA at GC tracts. This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Endo G; Endonuclease G, mitochondrial; mitochondrial endonuclease G
Gene Aliases: ENDOG
UniProt ID: (Human) Q14249, (Mouse) O08600
Entrez Gene ID: (Human) 2021, (Mouse) 13804, (Rat) 362100
in utero embryonic development
DNA metabolic process
DNA catabolic process
apoptotic DNA fragmentation
response to tumor necrosis factor
positive regulation of apoptotic process
response to antibiotic
nucleic acid phosphodiester bond hydrolysis
positive regulation of hydrogen peroxide-mediated programmed cell death
positive regulation of apoptotic DNA fragmentation
DNA recombination
aging
response to mechanical stimulus
response to estradiol
neuron death in response to oxidative stress
cellular response to calcium ion
cellular response to glucose stimulus
cellular response to hypoxia
cellular response to oxidative stress
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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