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ENO1 Polyclonal Antibody

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ENO1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 11204-1-AP (ENO1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ENO1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 11204-1-AP ( ENO1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

ENO1 Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11204-1-AP (ENO1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

ENO1 Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 0.2ug ENO1 antibody (11204-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

ENO1 Antibody in Immunoprecipitation (IP) — IP result of anti-ENO1 (IP:11204-1-AP, 3ug; Detection:11204-1-AP 1:500) with mouse skeletal muscle tissue lysate 3500ug.

Product Details

11204-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 19 publications 19 publications
Immunohistochemistry (IHC)	-	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:400	-
Immunocytochemistry (ICC/IF)	1:50-1:500	View 1 publication 1 publication
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ENO1 Fusion Protein Ag1692 (2-434 aa encoded by BC015641) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.27 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: SILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK (2-434 aa encoded by BC015641)

Target Information

Enolase (2-phosphogly-cerate hydrolyase or phosphopyruvate hydrates) is a glycolytic enzyme that catalyzes the dehydration and conversion of 2-phosphoglycerate to phosphoenolpyruvate. It comprises three distinct subunits, alpha, beta and gamma. Non Neuronal Enolase(ENO1) is an isoform of mammalian enolase and is composed of 2 alpha subunits. The gene for ENO1 also encodes a shorter monomeric structural lens protein, tau-crystallin. Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.Used as a diagnostic marker for many tumors and, in the heterodimeric form, alpha/gamma, as a marker for hypoxic brain injury after cardiac arrest. Also marker for endometriosis. Antibodies against alpha-enolase are present in sera from patients with cancer-associated retinopathy syndrome (CAR), a progressive blinding disease which occurs in the presence of systemic tumor growth, primarily small-cell carcinoma of the lung and other malignancies. Is identified as an autoantigen in Hashimoto encephalopathy (HE) a rare autoimmune disease associated with Hashimoto thyroiditis (HT). HT is a disorder in which destructive processes overcome the potential capacity of thyroid replacement leading to hypothyroidism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Enolase-1 serves as a biomarker of diagnosis and prognosis in hepatocellular carcinoma patients.

Cancer management and research

Zhu W,Li H,Yu Y,Chen J,Chen X,Ren F,Ren Z,Cui G

Wed Mar 30 00:00:00 EDT 2022

Quantitative Proteomic Profiling the Molecular Signatures of Annexin A5 in Lung Squamous Carcinoma Cells.

PloS one

Sun B,Bai Y,Zhang L,Gong L,Qi X,Li H,Wang F,Chi X,Jiang Y,Shao S

Wed Nov 20 00:00:00 EST 2019

Gamma-enolase predicts lung damage in severe acute pancreatitis-induced acute lung injury.

Journal of molecular histology

Owusu L,Xu C,Chen H,Liu G,Zhang G,Zhang J,Tang Z,Sun Z,Yi X

Wed Aug 01 00:00:00 EDT 2018

Upregulation of α-enolase protects cardiomyocytes from phenylephrine-induced hypertrophy.

Canadian journal of physiology and pharmacology

Gao S,Liu XP,Wei LH,Lu J,Liu P

Sun Apr 01 00:00:00 EDT 2018

Enolase 1 stimulates glycolysis to promote chemoresistance in gastric cancer.

Oncotarget

Qian X,Xu W,Xu J,Shi Q,Li J,Weng Y,Jiang Z,Feng L,Wang X,Zhou J,Jin H

Tue Jul 18 00:00:00 EDT 2017

Enolase 1 stimulates glycolysis to promote chemoresistance in gastric cancer.

Oncotarget

Qian X,Xu W,Xu J,Shi Q,Li J,Weng Y,Jiang Z,Feng L,Wang X,Zhou J,Jin H

Tue Jul 18 00:00:00 EDT 2017

Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.

The EMBO journal

Wang L,Zhang T,Wang L,Cai Y,Zhong X,He X,Hu L,Tian S,Wu M,Hui L,Zhang H,Gao P

Mon May 15 00:00:00 EDT 2017

CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.

EMBO reports

Zhong X,Tian S,Zhang X,Diao X,Dong F,Yang J,Li Z,Sun L,Wang L,He X,Wu G,Hu X,Wang L,Song L,Zhang H,Pan X,Li A,Gao P

Mon May 01 00:00:00 EDT 2017

Functional Effects of Cigarette Smoke-Induced Changes in Airway Smooth Muscle Mitochondrial Morphology.

Journal of cellular physiology

Aravamudan B,Thompson M,Sieck GC,Vassallo R,Pabelick CM,Prakash YS

Mon May 01 00:00:00 EDT 2017

Defining the Na⁺/H⁺ exchanger NHE1 interactome in triple-negative breast cancer cells.

Cellular signalling

Amith SR,Vincent KM,Wilkinson JM,Postovit LM,Fliegel L

Sun Jan 01 00:00:00 EST 2017

FBXW7 negatively regulates ENO1 expression and function in colorectal cancer.

Laboratory investigation; a journal of technical methods and pathology

Zhan P,Wang Y,Zhao S,Liu C,Wang Y,Wen M,Mao JH,Wei G,Zhang P

Tue Sep 01 00:00:00 EDT 2015

Enolase-1 is a therapeutic target in endometrial carcinoma.

Oncotarget

Zhao M,Fang W,Wang Y,Guo S,Shu L,Wang L,Chen Y,Fu Q,Liu Y,Hua S,Fan Y,Liu Y,Deng X,Luo R,Mei Z,Jiang Q,Liu Z

Sat Jun 20 00:00:00 EDT 2015

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Cell research

Sun L,Song L,Wan Q,Wu G,Li X,Wang Y,Wang J,Liu Z,Zhong X,He X,Shen S,Pan X,Li A,Wang Y,Gao P,Tang H,Zhang H

Wed Apr 01 00:00:00 EDT 2015

α-Enolase plays a catalytically independent role in doxorubicin-induced cardiomyocyte apoptosis and mitochondrial dysfunction.

Journal of molecular and cellular cardiology

Gao S,Li H,Feng XJ,Li M,Liu ZP,Cai Y,Lu J,Huang XY,Wang JJ,Li Q,Chen SR,Ye JT,Liu PQ

Sun Feb 01 00:00:00 EST 2015

Proteomic study reveals a co-occurrence of gallic acid-induced apoptosis and glycolysis in B16F10 melanoma cells.

Journal of agricultural and food chemistry

Liu C,Lin JJ,Yang ZY,Tsai CC,Hsu JL,Wu YJ

Wed Dec 03 00:00:00 EST 2014

Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.

Nature communications

Ma X,Li C,Sun L,Huang D,Li T,He X,Wu G,Yang Z,Zhong X,Song L,Gao P,Zhang H

Fri Oct 10 00:00:00 EDT 2014

Alpha-enolase as a potential cancer prognostic marker promotes cell growth, migration, and invasion in glioma.

Molecular cancer

Song Y,Luo Q,Long H,Hu Z,Que T,Zhang X,Li Z,Wang G,Yi L,Liu Z,Fang W,Qi S

Fri Mar 21 00:00:00 EDT 2014

Alpha-enolase as a potential cancer prognostic marker promotes cell growth, migration, and invasion in glioma.

Molecular cancer

Song Y,Luo Q,Long H,Hu Z,Que T,Zhang X,Li Z,Wang G,Yi L,Liu Z,Fang W,Qi S

Fri Mar 21 00:00:00 EDT 2014

Mitochondrial binding of α-enolase stabilizes mitochondrial membrane: its role in doxorubicin-induced cardiomyocyte apoptosis.

Archives of biochemistry and biophysics

Gao S,Li H,Cai Y,Ye JT,Liu ZP,Lu J,Huang XY,Feng XJ,Gao H,Chen SR,Li M,Liu PQ

Wed Jan 15 00:00:00 EST 2014

Mitochondrial binding of α-enolase stabilizes mitochondrial membrane: its role in doxorubicin-induced cardiomyocyte apoptosis.

Archives of biochemistry and biophysics

Gao S,Li H,Cai Y,Ye JT,Liu ZP,Lu J,Huang XY,Feng XJ,Gao H,Chen SR,Li M,Liu PQ

Wed Jan 15 00:00:00 EST 2014

Mitochondrial binding of α-enolase stabilizes mitochondrial membrane: its role in doxorubicin-induced cardiomyocyte apoptosis.

Archives of biochemistry and biophysics

Gao S,Li H,Cai Y,Ye JT,Liu ZP,Lu J,Huang XY,Feng XJ,Gao H,Chen SR,Li M,Liu PQ

Wed Jan 15 00:00:00 EST 2014

Gender-dimorphic regulation of skeletal muscle proteins in streptozotocin-induced diabetic rats.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Choi M,Choi JW,Chaudhari HN,Aseer KR,Mukherjee R,Yun JW

Mon Sep 16 00:00:00 EDT 2013

Identification of human host proteins contributing to H5N1 influenza virus propagation by membrane proteomics.

Journal of proteome research

Liu C,Zhang A,Guo J,Yang J,Zhou H,Chen H,Jin M

Fri Nov 02 00:00:00 EDT 2012

Proteomic features of potential tumor suppressor NESG1 in nasopharyngeal carcinoma.

Proteomics

Liu Z,Chen C,Yang H,Zhang Y,Long J,Long X,Fang W

Thu Nov 01 00:00:00 EDT 2012

Proteomic profiling of the 11-dehydrosinulariolide-treated oral carcinoma cells Ca9-22: effects on the cell apoptosis through mitochondrial-related and ER stress pathway.

Journal of proteomics

Liu CI,Wang RY,Lin JJ,Su JH,Chiu CC,Chen JC,Chen JY,Wu YJ

Mon Oct 22 00:00:00 EDT 2012

Proteomic analysis of the triglyceride-rich lipoprotein-laden foam cells.

Molecules and cells

Lu Y,Guo J,Di Y,Zong Y,Qu S,Tian J

Tue Sep 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: 2-phospho-D-glycerate hydro-lyase; 2-phospho-D-glycerate hydrolase; alpha enolase; alpha enolase like 1; Alpha-enolase; c-Myc promoter binding protein; C-myc promoter-binding protein; enoa; Enolase 1; enolase 1, (alpha); enolase 1, alpha non-neuron; enolase-alpha; epididymis secretory protein Li 17; MBP-1; MPB-1; MYC promoter-binding protein 1; NNE; Non-neural enolase; Phosphopyruvate hydratase; Plasminogen-binding protein; tau-crystallin

Gene Aliases: 0610008I15; AL022784; Eno-1; ENO1; ENO1L1; HEL-S-17; MBP-1; MBPB1; MPB1; NNE; PPH

UniProt ID: (Human) P06733, (Mouse) P17182, (Rat) P04764

Entrez Gene ID: (Human) 2023, (Mouse) 13806, (Rat) 24333

Molecular Function: lyase

Function(s)

negative regulation of transcription from RNA polymerase II promoter in utero embryonic development gluconeogenesis transcription, DNA-templated response to virus negative regulation of cell growth negative regulation of transcription, DNA-templated positive regulation of binding canonical glycolysis cell-cell adhesion cellular response to acid chemical glycolytic process

Process(es)

magnesium ion binding DNA binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity phosphopyruvate hydratase activity protein binding poly(A) RNA binding protein heterodimerization activity GTPase binding cadherin binding involved in cell-cell adhesion RNA binding protein homodimerization activity

ENO1 Polyclonal Antibody

Product Details

11204-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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