Proteintech

ENT1 Polyclonal Antibody

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ENT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 11337-1-AP (ENT1 antibody) at dilution of 1:100 (under 10x lens).

ENT1 Antibody in Western Blot (WB) — Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11337-1-AP (ENT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ENT1 Antibody in Immunoprecipitation (IP) — IP result of anti-ENT1 (IP:11337-1-AP, 3ug; Detection:11337-1-AP 1:400) with mouse brain tissue lysate 8000ug.

Product Details

11337-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 10 publications 10 publications
Immunohistochemistry (IHC)	-	View 9 publications 9 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ENT1 Fusion Protein Ag1881 (150-456 aa encoded by BC008954) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.16 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: INSFGAILQ GSLFGLAGLL PASYTAPIMS GQGLAGFFAS VAMICAIASG SELSESAFGY FITACAVIIL TIICYLGLPR LEFYRYYQQL KLEGPGEQET KLDLISKGEE PRAGKEESGV SVSNSQPTNE SHSIKAILKN ISVLAFSVCF IFTITIGMFP AVTVEVKSSI AGSSTWERYF IPVSCFLTFN IFDWLGRSLT AVFMWPGKDS RWLPSLVLAR LVFVPLLLLC NIKPRRYLTV VFEHDAWFIF FMAAFAFSNG YLASLCMCFG PKKVKPAEAE TAGAIMAFFL CLGLALGAVF SFLFRAIV (150-456 aa encoded by BC008954)

Target Information

This gene is a member of the equilibrative nucleoside transporter family. The gene encodes a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative transporter that is sensitive to inhibition by nitrobenzylthioinosine. Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Abrogation of glutathione peroxidase-1 drives EMT and chemoresistance in pancreatic cancer by activating ROS-mediated Akt/GSK3β/Snail signaling.

Oncogene

Meng Q,Shi S,Liang C,Liang D,Hua J,Zhang B,Xu J,Yu X

Thu Nov 01 00:00:00 EDT 2018

Abrogation of glutathione peroxidase-1 drives EMT and chemoresistance in pancreatic cancer by activating ROS-mediated Akt/GSK3β/Snail signaling.

Oncogene

Meng Q,Shi S,Liang C,Liang D,Hua J,Zhang B,Xu J,Yu X

Thu Nov 01 00:00:00 EDT 2018

Correlation of 4'-[methyl-¹¹C]-thiothymidine uptake with human equilibrative nucleoside transporter-1 and thymidine kinase-1 expressions in patients with newly diagnosed gliomas.

Annals of nuclear medicine

Takami Y,Yamamoto Y,Ueno M,Chiba Y,Norikane T,Hatakeyama T,Miyake K,Toyohara J,Nishiyama Y

Thu Nov 01 00:00:00 EDT 2018

Bortezomib prevents cytarabine resistance in MCL, which is characterized by down-regulation of dCK and up-regulation of SPIB resulting in high NF-κB activity.

BMC cancer

Freiburghaus C,Emruli VK,Johansson A,Eskelund CW,Grønbæk K,Olsson R,Ek F,Jerkeman M,Ek S

Wed Apr 25 00:00:00 EDT 2018

FBW7 increases the chemosensitivity of pancreatic cancer cells to gemcitabine through upregulation of ENT1.

Oncology reports

Hu Q,Qin Y,Zhang B,Liang C,Ji S,Shi S,Xu W,Xiang J,Liang D,Ni Q,Yu X,Xu J

Sun Oct 01 00:00:00 EDT 2017

FBW7 increases the chemosensitivity of pancreatic cancer cells to gemcitabine through upregulation of ENT1.

Oncology reports

Hu Q,Qin Y,Zhang B,Liang C,Ji S,Shi S,Xu W,Xiang J,Liang D,Ni Q,Yu X,Xu J

Sun Oct 01 00:00:00 EDT 2017

Deficient Insulin-mediated Upregulation of the Equilibrative Nucleoside Transporter 2 Contributes to Chronically Increased Adenosine in Diabetic Glomerulopathy.

Scientific reports

Alarcón S,Garrido W,Vega G,Cappelli C,Suárez R,Oyarzún C,Quezada C,San Martín R

Fri Aug 25 00:00:00 EDT 2017

Reduced Adenosine Uptake and Its Contribution to Signaling that Mediates Profibrotic Activation in Renal Tubular Epithelial Cells: Implication in Diabetic Nephropathy.

PloS one

Kretschmar C,Oyarzún C,Villablanca C,Jaramillo C,Alarcón S,Perez G,Díaz-Encarnación MM,Pastor-Anglada M,Garrido W,Quezada C,San Martín R

Fri Jul 08 00:00:00 EDT 2016

Reduced Adenosine Uptake and Its Contribution to Signaling that Mediates Profibrotic Activation in Renal Tubular Epithelial Cells: Implication in Diabetic Nephropathy.

PloS one

Kretschmar C,Oyarzún C,Villablanca C,Jaramillo C,Alarcón S,Perez G,Díaz-Encarnación MM,Pastor-Anglada M,Garrido W,Quezada C,San Martín R

Fri Jul 08 00:00:00 EDT 2016

p21-Activated Kinase 4 (PAK4) as a Predictive Marker of Gemcitabine Sensitivity in Pancreatic Cancer Cell Lines.

Cancer research and treatment

Moon SU,Kim JW,Sung JH,Kang MH,Kim SH,Chang H,Lee JO,Kim YJ,Lee KW,Kim JH,Bang SM,Lee JS

Wed Jul 01 00:00:00 EDT 2015

The role of human equilibrative nucleoside transporter 1 on the cellular transport of the DNA methyltransferase inhibitors 5-azacytidine and CP-4200 in human leukemia cells.

Molecular pharmacology

Hummel-Eisenbeiss J,Hascher A,Hals PA,Sandvold ML,Müller-Tidow C,Lyko F,Rius M

Sun Sep 01 00:00:00 EDT 2013

Prognostic predictive values of gemcitabine sensitivity-related gene products for unresectable or recurrent biliary tract cancer treated with gemcitabine alone.

World journal of surgical oncology

Murata A,Amano R,Yamada N,Kimura K,Yashiro M,Nakata B,Hirakawa K

Mon May 27 00:00:00 EDT 2013

Expression of equilibrative nucleoside transporter 1 in rat circumvallate papillae.

Neuroscience letters

Nishida K,Kitada T,Kato J,Dohi Y,Nagasawa K

Tue Jan 15 00:00:00 EST 2013

Human equilibrative nucleoside transporter 1 level does not predict prognosis in pancreatic cancer patients treated with neoadjuvant chemoradiation including gemcitabine.

Journal of hepato-biliary-pancreatic sciences

Kawada N,Uehara H,Katayama K,Nakamura S,Takahashi H,Ohigashi H,Ishikawa O,Nagata S,Tomita Y

Thu Nov 01 00:00:00 EDT 2012

Peroxynitrite treatment reduces adenosine uptake via the equilibrative nucleoside transporter in rat astrocytes.

Neuroscience letters

Tanaka A,Nishida K,Okuda H,Nishiura T,Higashi Y,Fujimoto S,Nagasawa K

Fri Jul 01 00:00:00 EDT 2011

Peroxynitrite treatment reduces adenosine uptake via the equilibrative nucleoside transporter in rat astrocytes.

Neuroscience letters

Tanaka A,Nishida K,Okuda H,Nishiura T,Higashi Y,Fujimoto S,Nagasawa K

Fri Jul 01 00:00:00 EDT 2011

The prognostic significance of human equilibrative nucleoside transporter 1 expression in patients with metastatic bladder cancer treated with gemcitabine-cisplatin-based combination chemotherapy.

BJU international

Matsumura N,Nakamura Y,Kohjimoto Y,Inagaki T,Nanpo Y,Yasuoka H,Ohashi Y,Hara I

Fri Jul 01 00:00:00 EDT 2011

Contribution of P2X7 receptors to adenosine uptake by cultured mouse astrocytes.

Glia

Okuda H,Higashi Y,Nishida K,Fujimoto S,Nagasawa K

Mon Nov 01 00:00:00 EDT 2010

Contribution of thymidylate synthase to gemcitabine therapy for advanced pancreatic cancer.

Pancreas

Komori S,Osada S,Mori R,Matsui S,Sanada Y,Tomita H,Tokuyama Y,Takahashi T,Yamaguchi K,Yoshida K

Mon Nov 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: Equilibrative NBMPR-sensitive nucleoside transporter; equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter; Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter; Equilibrative nucleoside transporter 1; equilibrative nucleoside transporter 1 variant delta 11; HENT1; NBMPR-sensitive equilibrative nucleoside transporter; Nucleoside transporter, es-type; solute carrier family 29 (equilibrative nucleoside transporter), member 1; solute carrier family 29 (nucleoside transporters), member 1; Solute carrier family 29 member 1; solute carrier family 29, member 1

Gene Aliases: 1200014D21Rik; AA407560; ENT1; rENT1; SLC29A1

UniProt ID: (Human) Q99808, (Mouse) Q9JIM1, (Rat) O54698

Entrez Gene ID: (Human) 2030, (Mouse) 63959, (Rat) 63997

Molecular Function: transporter