Proteintech

ENT1 Polyclonal Antibody

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ENT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 11337-1-AP (ENT1 antibody) at dilution of 1:100 (under 10x lens).

ENT1 Antibody in Western Blot (WB) — Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11337-1-AP (ENT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ENT1 Antibody in Immunoprecipitation (IP) — IP result of anti-ENT1 (IP:11337-1-AP, 3ug; Detection:11337-1-AP 1:400) with mouse brain tissue lysate 8000ug.

Product Details

11337-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 19 publications 19 publications

Immunohistochemistry (IHC)

View 13 publications 13 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ENT1 Fusion Protein Ag1881 (150-456 aa encoded by BC008954)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.16 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: INSFGAILQ GSLFGLAGLL PASYTAPIMS GQGLAGFFAS VAMICAIASG SELSESAFGY FITACAVIIL TIICYLGLPR LEFYRYYQQL KLEGPGEQET KLDLISKGEE PRAGKEESGV SVSNSQPTNE SHSIKAILKN ISVLAFSVCF IFTITIGMFP AVTVEVKSSI AGSSTWERYF IPVSCFLTFN IFDWLGRSLT AVFMWPGKDS RWLPSLVLAR LVFVPLLLLC NIKPRRYLTV VFEHDAWFIF FMAAFAFSNG YLASLCMCFG PKKVKPAEAE TAGAIMAFFL CLGLALGAVF SFLFRAIV (150-456 aa encoded by BC008954)

Target Information

This gene is a member of the equilibrative nucleoside transporter family. The gene encodes a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative transporter that is sensitive to inhibition by nitrobenzylthioinosine. Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Abrogation of ARF6 promotes RSL3-induced ferroptosis and mitigates gemcitabine resistance in pancreatic cancer cells.

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ZIP4 Increases Expression of Transcription Factor ZEB1 to Promote Integrin α3β1 Signaling and Inhibit Expression of the Gemcitabine Transporter ENT1 in Pancreatic Cancer Cells.

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Predictive and Prognostic Properties of Human Equilibrative Nucleoside Transporter 1 Expression in Gemcitabine-Treated Pancreatobiliary Cancer: A Meta-Analysis.

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Roles of organic anion transporter 2 and equilibrative nucleoside transporter 1 in hepatic disposition and antiviral activity of entecavir during non-pregnancy and pregnancy.

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Correlation of 4'-[methyl-¹¹C]-thiothymidine uptake with human equilibrative nucleoside transporter-1 and thymidine kinase-1 expressions in patients with newly diagnosed gliomas.

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Correlation of 4'-[methyl-¹¹C]-thiothymidine uptake with human equilibrative nucleoside transporter-1 and thymidine kinase-1 expressions in patients with newly diagnosed gliomas.

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Abrogation of glutathione peroxidase-1 drives EMT and chemoresistance in pancreatic cancer by activating ROS-mediated Akt/GSK3β/Snail signaling.

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Abrogation of glutathione peroxidase-1 drives EMT and chemoresistance in pancreatic cancer by activating ROS-mediated Akt/GSK3β/Snail signaling.

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Abrogation of glutathione peroxidase-1 drives EMT and chemoresistance in pancreatic cancer by activating ROS-mediated Akt/GSK3β/Snail signaling.

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FBW7 increases the chemosensitivity of pancreatic cancer cells to gemcitabine through upregulation of ENT1.

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Reduced Adenosine Uptake and Its Contribution to Signaling that Mediates Profibrotic Activation in Renal Tubular Epithelial Cells: Implication in Diabetic Nephropathy.

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Kretschmar C,Oyarzún C,Villablanca C,Jaramillo C,Alarcón S,Perez G,Díaz-Encarnación MM,Pastor-Anglada M,Garrido W,Quezada C,San Martín R

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Reduced Adenosine Uptake and Its Contribution to Signaling that Mediates Profibrotic Activation in Renal Tubular Epithelial Cells: Implication in Diabetic Nephropathy.

PloS one

Kretschmar C,Oyarzún C,Villablanca C,Jaramillo C,Alarcón S,Perez G,Díaz-Encarnación MM,Pastor-Anglada M,Garrido W,Quezada C,San Martín R

Fri Jul 08 00:00:00 EDT 2016

Reduced Adenosine Uptake and Its Contribution to Signaling that Mediates Profibrotic Activation in Renal Tubular Epithelial Cells: Implication in Diabetic Nephropathy.

PloS one

Kretschmar C,Oyarzún C,Villablanca C,Jaramillo C,Alarcón S,Perez G,Díaz-Encarnación MM,Pastor-Anglada M,Garrido W,Quezada C,San Martín R

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Prognostic predictive values of gemcitabine sensitivity-related gene products for unresectable or recurrent biliary tract cancer treated with gemcitabine alone.

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Expression of equilibrative nucleoside transporter 1 in rat circumvallate papillae.

Neuroscience letters

Nishida K,Kitada T,Kato J,Dohi Y,Nagasawa K

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Kawada N,Uehara H,Katayama K,Nakamura S,Takahashi H,Ohigashi H,Ishikawa O,Nagata S,Tomita Y

Thu Nov 01 00:00:00 EDT 2012

Human equilibrative nucleoside transporter 1 level does not predict prognosis in pancreatic cancer patients treated with neoadjuvant chemoradiation including gemcitabine.

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The prognostic significance of human equilibrative nucleoside transporter 1 expression in patients with metastatic bladder cancer treated with gemcitabine-cisplatin-based combination chemotherapy.

BJU international

Matsumura N,Nakamura Y,Kohjimoto Y,Inagaki T,Nanpo Y,Yasuoka H,Ohashi Y,Hara I

Fri Jul 01 00:00:00 EDT 2011

Peroxynitrite treatment reduces adenosine uptake via the equilibrative nucleoside transporter in rat astrocytes.

Neuroscience letters

Tanaka A,Nishida K,Okuda H,Nishiura T,Higashi Y,Fujimoto S,Nagasawa K

Fri Jul 01 00:00:00 EDT 2011

Peroxynitrite treatment reduces adenosine uptake via the equilibrative nucleoside transporter in rat astrocytes.

Neuroscience letters

Tanaka A,Nishida K,Okuda H,Nishiura T,Higashi Y,Fujimoto S,Nagasawa K

Fri Jul 01 00:00:00 EDT 2011

Peroxynitrite treatment reduces adenosine uptake via the equilibrative nucleoside transporter in rat astrocytes.

Neuroscience letters

Tanaka A,Nishida K,Okuda H,Nishiura T,Higashi Y,Fujimoto S,Nagasawa K

Fri Jul 01 00:00:00 EDT 2011

Peroxynitrite treatment reduces adenosine uptake via the equilibrative nucleoside transporter in rat astrocytes.

Neuroscience letters

Tanaka A,Nishida K,Okuda H,Nishiura T,Higashi Y,Fujimoto S,Nagasawa K

Fri Jul 01 00:00:00 EDT 2011

Contribution of P2X7 receptors to adenosine uptake by cultured mouse astrocytes.

Glia

Okuda H,Higashi Y,Nishida K,Fujimoto S,Nagasawa K

Mon Nov 01 00:00:00 EDT 2010

Contribution of P2X7 receptors to adenosine uptake by cultured mouse astrocytes.

Glia

Okuda H,Higashi Y,Nishida K,Fujimoto S,Nagasawa K

Mon Nov 01 00:00:00 EDT 2010

Contribution of thymidylate synthase to gemcitabine therapy for advanced pancreatic cancer.

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Komori S,Osada S,Mori R,Matsui S,Sanada Y,Tomita H,Tokuyama Y,Takahashi T,Yamaguchi K,Yoshida K

Mon Nov 01 00:00:00 EDT 2010

Contribution of thymidylate synthase to gemcitabine therapy for advanced pancreatic cancer.

Pancreas

Komori S,Osada S,Mori R,Matsui S,Sanada Y,Tomita H,Tokuyama Y,Takahashi T,Yamaguchi K,Yoshida K

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Bioinformatics

Protein Aliases: Equilibrative NBMPR-sensitive nucleoside transporter; equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter; Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter; Equilibrative nucleoside transporter 1; equilibrative nucleoside transporter 1 variant delta 11; HENT1; NBMPR-sensitive equilibrative nucleoside transporter; Nucleoside transporter, es-type; solute carrier family 29 (equilibrative nucleoside transporter), member 1; solute carrier family 29 (nucleoside transporters), member 1; Solute carrier family 29 member 1; solute carrier family 29, member 1

Gene Aliases: 1200014D21Rik; AA407560; ENT1; rENT1; SLC29A1

UniProt ID: (Human) Q99808, (Mouse) Q9JIM1, (Rat) O54698

Entrez Gene ID: (Human) 2030, (Mouse) 63959, (Rat) 63997

Molecular Function: transporter