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Invitrogen
ENT1 Polyclonal Antibody
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FIGURE: 1 / 1
ENT1 Antibody (PA5-89074) in WB
Western blot analysis of ENT1 in extracts of U-87MG cells. Samples were incubated with ENT1 Polyclonal antibody (Product # PA5-89074) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-89074
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human SLC29A1 (NP_0010716451)
Conjugate
Form
Liquid
Concentration
1.31 mg/mL
Amount
131 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: ELSESAFGYF ITACAVIILT IICYLGLPRL EFYRYYQQLK LEGPGEQETK LDLISKGEEP RAGKEESGVS VSNSQPTNES HSIKAILKNI SVLAFSVCFI F; Positive Samples: U-87MG; Cellular Location: Apical cell membrane, Basolateral cell membrane, Cell membrane, Multi-pass membrane protein
Target Information
This gene is a member of the equilibrative nucleoside transporter family. The gene encodes a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative transporter that is sensitive to inhibition by nitrobenzylthioinosine. Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Equilibrative NBMPR-sensitive nucleoside transporter; equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter; Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter; Equilibrative nucleoside transporter 1; es nucleoside transporter; HENT1; Nucleoside transporter, es-type; solute carrier family 29 (equilibrative nucleoside transporter), member 1; solute carrier family 29 (nucleoside transporters), member 1; Solute carrier family 29 member 1; unnamed protein product
Gene Aliases: AUG; ENT1; hENT1; SLC29A1
UniProt ID: (Human) Q99808
Entrez Gene ID: (Human) 2030
neurotransmitter transporter activity
nucleoside transmembrane transporter activity
adenine transmembrane transporter activity
guanine transmembrane transporter activity
uracil transmembrane transporter activity
purine nucleoside transmembrane transporter activity
cytidine transmembrane transporter activity
uridine transmembrane transporter activity
pyrimidine- and adenine-specific:sodium symporter activity
neurotransmitter uptake
nucleobase-containing compound metabolic process
xenobiotic metabolic process
neurotransmitter transport
drug transmembrane transport
lactation
nucleobase transport
adenine transport
nucleoside transport
purine nucleoside transmembrane transport
cytidine transport
uridine transport
adenosine transport
inosine transport
hypoxanthine transport
thymine transport
excitatory postsynaptic potential
cellular response to glucose stimulus
cellular response to hypoxia
pyrimidine-containing compound transmembrane transport
transport across blood-brain barrier
nucleoside transmembrane transport
guanine transmembrane transport
uracil transmembrane transport
pyrimidine nucleobase transmembrane transport
purine nucleobase transmembrane transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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