Invitrogen

ENTPD5 Polyclonal Antibody

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ENTPD5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunocytochemical analysis of paraffin-embeded ENTPD5 in human small intestine tissue using a ENTPD5 Polyclonal antibody (Product # PA5-112412) at a dilution of 1:100.

ENTPD5 Antibody in Western Blot (WB) — Western Blot analysis of ENTPD5 in multiple sample types using a ENTPD5 polyclonal antibody (Product # PA5-112412) at a concentration of 4 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:10,000. Sample types that were tested include: Lane 1: Mouse liver tissue, Lane 2: Mouse kidney tissue, Lane 3: Mouse gonad tissue.

ENTPD5 Antibody in Immunoprecipitation (IP) — Immunoprecipitation analysis of ENTPD5 in Hela whole cell lysate using a Polyclonal antibody (Product #PA5-112412). Samples were treated with 4 µg of the primary antibody and an HRP-conjugated anti-rabbit IgG secondary antibody was used at a dilution of 1:50,000. Lane 1: Rabbit control IgG, Lane 2: 500 µg of Hela whole cell lysate, Lane 3: 20 µg of Hela whole cell lysate.

Product Details

PA5-112412

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:5,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
ELISA (ELISA)	Assay-dependent	-
Immunoprecipitation (IP)	1:200-1:2,000	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant Human Ectonucleoside triphosphate diphosphohydrolase 5 protein (149-428AA) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.38 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C or -80°C if preferred
RRID	AB_2867147

Target Information

The protein encoded by this gene is similar to E-type nucleotidases (NTPases)/ecto-ATPase/apyrases. NTPases, such as CD39, mediate catabolism of extracellular nucleotides. ENTPD5 contains 4 apyrase-conserved regions which is characteristic of NTPases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD39 antigen-like 4; CD39-like 4; Ectonucleoside triphosphate diphosphohydrolase 5; ER-UDPase; GDPase ENTPD5; Guanosine-diphosphatase ENTPD5; Inosine diphosphate phosphatase ENTPD5; NTPDase 5; Nucleoside diphosphatase; Nucleoside diphosphate phosphatase ENTPD5; Pcph proto-oncogene protein; proto-oncogene CPH; UDPase ENTPD5; Uridine-diphosphatase ENTPD5

Gene Aliases: AI196558; AI987697; CD39L4; ENTPD5; ER-UDPase; mNTPase; NTPDase-5; NTPDase5; PCPH

UniProt ID: (Human) O75356, (Mouse) Q9WUZ9

Entrez Gene ID: (Human) 957, (Mouse) 12499

Molecular Function: hydrolase nucleotide phosphatase phosphatase metabolite interconversion enzyme