Western blot analysis of ENY2 using A) 30 µg NT2D1 whole cell lysate (B) 30 µg PC-3 whole cell lysate (C) 30 µg U87-MG whole cell lysate and D) 30 µg SK-N-SH whole cell lysate. Samples were loaded onto a 15% SDS-PAGE gel and probed with an ENY2 polyclonal antibody (Product # PA5-34959) at a dilution of 1:1000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment contains a sequence corresponding to a region within amino acids 1 and 101 of ENY2|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34959 targets ENY2 in WB applications and shows reactivity with Human samples.
The PA5-34959 immunogen is recombinant fragment contains a sequence corresponding to a region within amino acids 1 and 101 of ENY2.
Component of the transcription regulatory histone acetylation (HAT) complex SAGA, a multiprotein complex that activates transcription by remodeling chromatin and mediating histone acetylation and deubiquitination. Within the SAGA complex, participates in a subcomplex that specifically deubiquitinates both histones H2A and H2B. The SAGA complex is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation. May also participate in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery .
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.