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Invitrogen
EPN2 Polyclonal Antibody
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FIGURE: 1 / 1
EPN2 Antibody (PA5-109689) in WB
Western blot analysis of EPN2 in extracts of HepG2 cells. Samples were incubated with EPN2 Polyclonal antibody (Product # PA5-109689) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-109689
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of mouse Epn2 (XP_006532226.1).
Conjugate
Form
Liquid
Concentration
0.27 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: IFAIQTLKDF QYIDRDGKDQ GINVREKSKQ LVALLKDEER LKVERVQALK TKERMAQVAT GVGSNQITFG RGSSQPNLST SYSEQEYGKA GGSPASYHGS P
Target Information
Epsin2 plays a role in the formation of clathrin-coated invaginations and endocytosis. It binds to EPS15 (via the NPF repeat domain), as well as to AP-2 and clathrin (via the DPW repeat domain). The protein resides in the cytoplasm, in punctate structures throughout the cell, associated with clathrin-coated vesicles, and is particularly concentrated in the region of the Golgi complex. Highest expression is found in brain, with lower levels detected in lung and liver.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EH domain binding protein epsin 2; EPS-15-interacting protein 2; Eps15 binding protein; Epsin-2; Ibp2; Intersectin-EH-binding protein 2
Gene Aliases: 9530051D10Rik; AA536924; EHB21; EPN2; Ibp2; KIAA1065
UniProt ID: (Human) O95208, (Mouse) Q8CHU3
Entrez Gene ID: (Human) 22905, (Mouse) 13855, (Rat) 60443
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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