Invitrogen

EPRS Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (20) EPRS antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (4)
Advanced Verification (1)

EPRS Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 5

EPRS Antibody (PA5-56323) in IHC

Immunohistochemical staining of EPRS in human cerebral cortex and pancreas tissues using EPRS Polyclonal Antibody (Product # PA5-56323). Corresponding EPRS RNA-seq data are presented for the same tissues.

Product Image Gallery

EPRS Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of EPRS in human cell line U-2 OS shows positivity in cytoplasm. Samples were probed using an EPRS Polyclonal Antibody (Product # PA5-56323).

EPRS Antibody in Western Blot (WB) — Western blot analysis of EPRS using EPRS Polyclonal Antibody (Product # PA5-56323) (A) shows similar pattern to an independent EPRS Polyclonal Antibody (B).

EPRS Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of EPRS demonstrates antibody specificity. Immunohistochemical analysis of EPRS using anti-EPRS Polyclonal Antibody (Product # PA5-56323), shows significant staining of EPRS in human cerebral cortex and shows minimal or weak staining in human pancreas tissues. The relative expression levels of EPRS within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-56323

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human EPRS

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2641072

Product Specific Information

Immunogen sequence: DWYSQVITKS EMIEYHDISG CYILRPWAYA IWEAIKDFFD AEIKKLGVEN CYFPMFVSQS ALEKEKTHVA DFAPEVAWVT RSGKTELA

Highest antigen sequence identity to the following orthologs: Mouse - 88%, Rat - 89%.

Target Information

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. EPRS is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Bifunctional aminoacyl-tRNA synthetase; Bifunctional glutamate/proline--tRNA ligase; Cell proliferation-inducing gene 32 protein; GluRS; glutamate tRNA ligase; Glutamate--tRNA ligase; Glutamatyl-prolyl-tRNA synthetase; glutaminyl-tRNA synthetase; Glutamyl-tRNA synthetase; proliferation-inducing gene 32 protein; proliferation-inducing protein 32; proline tRNA ligase; Proline--tRNA ligase; proline-tRNA ligase; Prolyl-tRNA synthetase

Gene Aliases: EARS; EPRS; EPRS1; GLNS; GLUPRORS; PARS; PIG32; QARS; QPRS

UniProt ID: (Human) P07814

Entrez Gene ID: (Human) 2058

Molecular Function: aminoacyl-tRNA synthetase