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EPRS Antibody (PA5-90316) in WB
Western blot analysis of EPRS in extracts of various cell lines. Samples were incubated with EPRS Polyclonal antibody (Product # PA5-90316) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-90316
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 900-1000 of human EPRS (NP_0044372)
Conjugate
Form
Liquid
Concentration
1.22 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806022
Product Specific Information
Immunogen sequence: EAKVLFDKVA SQGEVVRKLK TEKAPKDQVD IAVQELLQLK AQYKSLIGVE YKPVSATGAE DKDKKKKEKE NKSEKQNKPQ KQNDGQRKDP SKNQGGGLSS S; Positive Samples: 293T, HeLa; Cellular Location: Cytoplasm
Target Information
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. EPRS is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Bifunctional aminoacyl-tRNA synthetase; Bifunctional glutamate/proline--tRNA ligase; Cell proliferation-inducing gene 32 protein; glutamate tRNA ligase; Glutamatyl-prolyl-tRNA synthetase; glutaminyl-tRNA synthetase; proliferation-inducing gene 32 protein; proliferation-inducing protein 32; proline tRNA ligase; proline-tRNA ligase; prolyl-tRNA synthetase
Gene Aliases: EARS; EPRS; EPRS1; GLNS; GLUPRORS; PARS; PIG32; QARS; QPRS
UniProt ID: (Human) P07814
Entrez Gene ID: (Human) 2058
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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