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EPS15R Antibody (PA5-102878) in ICC/IF
Immunocytochemistry analysis of EPS15R in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with EPS15R polyclonal antibody (Product # PA5-102878) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-102878
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human EPS15L1(Accession Q9UBC2), corresponding to amino acid residues Q746-S796.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2852261
Product Specific Information
Antibody detects endogenous levels of total EPS15L1.
Target Information
Seems to be a constitutive component of clathrin-coated pits that is required for receptor-mediated endocytosis. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epidermal growth factor receptor pathway substrate 15-like 1; Epidermal growth factor receptor pathway substrate 15-related sequence; Epidermal growth factor receptor substrate 15-like 1; epidermal growth factor receptor substrate EPS15R; Eps15-related protein; Eps15-rs; Eps15R
Gene Aliases: 9830147J04Rik; AI593686; Eps15-rs; EPS15L1; EPS15R
UniProt ID: (Human) Q9UBC2, (Mouse) Q60902
Entrez Gene ID: (Human) 58513, (Mouse) 13859, (Rat) 361120
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