Proteintech

EPS8 Polyclonal Antibody

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Antibody Testing Data (9)

FIGURE: 1 / 9

EPS8 Antibody (12455-1-AP) in WB

Various lysates were subjected to SDS PAGE followed by western blot with (Product # 12455-1-AP) EPS8 antibody at dilution of 1:1,000 incubated at room temperature for 1.5 hours.

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EPS8 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of A375 cells using 12455-1-AP (EPS8 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

EPS8 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 12455-1-AP ( EPS8 antibody) at dilution of 1:100 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

EPS8 Antibody in Immunoprecipitation (IP) — IP result of anti-EPS8 (IP:12455-1-AP, 3ug; Detection:12455-1-AP 1:500) with HeLa cells lysate 3800ug.

Product Details

12455-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:10-1:100

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

EPS8 Fusion Protein Ag3121 (13-267 aa encoded by BC030010)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.49 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: GMYPSQMNG YGSSPTFSQT DREHGSKTSA KALYEQRKNY ARDSVSSVSD ISQYRVEHLT TFVLDRKDAM ITVDDGIRKL KLLDAKGKVW TQDMILQVDD RAVSLIDLES KNELENFPLN TIQHCQAVMH SCSYDSVLAL VCKEPTQNKP DLHLFQCDEV KANLISEDIE SAISDSKGGK QKRRPDALRM ISNADPSIPP PPRAPAPAPP GTVTQVDVRS RVAAWSAWAA DQGDFEKPRQ YHEQEETPEM MAARID (13-267 aa encoded by BC030010)

Target Information

This gene encodes a member of the EPS8 family. This protein contains one PH domain and one SH3 domain. It functions as part of the EGFR pathway, though its exact role has not been determined. Highly similar proteins in other organisms are involved in the transduction of signals from Ras to Rac and growth factor-mediated actin remodeling. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.