Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 9
ERAL1 Antibody (PA5-99997) in ICC/IF
Immunofluorescent analysis of ERAL1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and ERAL1 polyclonal antibody (Product # PA5-99997) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-99997
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ERAL1(Accession O75616), corresponding to amino acid residues L101-Q151.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2818930
Product Specific Information
Antibody detects endogenous levels of total ERAL1.
Target Information
The protein encoded by this gene is a GTPase that localizes to the mitochondrion. The encoded protein binds to the 3' terminal stem loop of 12S mitochondrial rRNA and is required for proper assembly of the 28S small mitochondrial ribosomal subunit. Deletion of this gene has been shown to cause mitochondrial dysfunction, growth retardation, and apoptosis. Several transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: CEGA; Conserved ERA-like GTPase; era (E. coli G-protein homolog)-like 1; Era G-protein-like 1; Era-like 12S mitochondrial rRNA chaperone 1; ERA-like GTPase; ERA-like protein 1; ERA-W; GTP-binding protein era homolog; GTPase Era, mitochondrial; GTPase, human homolog of E. coli essential cell cycle protein Era; H-ERA; M-ERA
Gene Aliases: 2610524P08Rik; 9130407C09Rik; AU019798; CEGA; ERA; ERA-W; ERAL1; ERAL1A; H-ERA; HERA; HERA-A; HERA-B; M-ERA; Mera; MERA-S; MERA-W
UniProt ID: (Human) O75616, (Mouse) Q9CZU4
Entrez Gene ID: (Human) 26284, (Mouse) 57837
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support