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Invitrogen
ERCC1 Polyclonal Antibody
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FIGURE: 1 / 8
ERCC1 Antibody (PA5-77971) in ICC/IF
Immunofluorescence analysis of ERCC1 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ERCC1 Polyclonal Antibody (Product # PA5-77971) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a di... View More
Product Details
PA5-77971
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human ERCC1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: 293T, A431, HeLa, HepG2, PC-12, Rat2
Predicted Reactivity: Mouse (86%), Pig (87%), Chimpanzee (100%), Bovine (87%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ERCC1 is a non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. It is responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). It participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. ERCC1 is also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4. (Uniprot)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: clone 2B3; DNA excision repair protein ERCC-1; excision repair cross-complementation group 1; excision repair cross-complementing 1; excision repair cross-complementing rodent repair deficiency, complementation group 1; excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence); unnamed protein product
Gene Aliases: COFS4; Ercc-1; ERCC1; RAD10; UV20
UniProt ID: (Human) P07992, (Mouse) P07903
Entrez Gene ID: (Human) 2067, (Rat) 292673, (Mouse) 13870
single-stranded DNA endodeoxyribonuclease activity
TFIID-class transcription factor binding
DNA binding
damaged DNA binding
single-stranded DNA binding
protein binding
3' overhang single-stranded DNA endodeoxyribonuclease activity
promoter-specific chromatin binding
molecular_function
protein C-terminus binding
TBP-class protein binding
protein domain specific binding
structure-specific DNA binding
nuclease activity
endonuclease activity
hydrolase activity
endodeoxyribonuclease
pyrimidine dimer repair by nucleotide-excision repair
telomere maintenance
DNA repair
nucleotide-excision repair
double-strand break repair
double-strand break repair via nonhomologous end joining
DNA recombination
mitotic recombination
syncytium formation
cellular response to DNA damage stimulus
response to oxidative stress
germ cell development
spermatogenesis
cell proliferation
determination of adult lifespan
male gonad development
UV protection
response to X-ray
regulation of telomere maintenance
negative regulation of telomere maintenance
post-embryonic hemopoiesis
multicellular organism growth
interstrand cross-link repair
isotype switching
insulin-like growth factor receptor signaling pathway
cell development
oogenesis
chromosome organization
positive regulation of transcription initiation from RNA polymerase II promoter
telomeric DNA-containing double minutes formation
UV-damage excision repair
replicative senescence
t-circle formation
positive regulation of t-circle formation
negative regulation of protection from non-homologous end joining at telomere
DNA catabolic process, endonucleolytic
replicative cell aging
nucleotide-excision repair, DNA incision, 3'-to lesion
nucleotide-excision repair, DNA incision, 5'-to lesion
response to nutrient
response to sucrose
multicellular organism aging
embryonic organ development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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