Invitrogen

ERCC1 Polyclonal Antibody

Product Image Gallery

ERCC1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of ERCC1 in A431 cells. Samples were treated with 4% paraformaldehyde at RT for 15 min and incubated with ERCC1 polyclonal antibody (Product # PA5-77971) using a dilution of 1:1000, followed by Hoechst.

ERCC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of ERCC1 in paraffin-embedded human lung carcinoma. Sample was incubated with ERCC1 polyclonal antibody (Product # PA5-77971) using a dilution of 1:250.

ERCC1 Antibody in Western Blot (WB) — Western blot analysis of ERCC1 in A) 293T whole cell lysate, B) A431 whole cell lysate, C) HeLa whole cell lysate, D) HepG2 whole cell lysate using 30 µg of protein. Samples were separated with 12% SDS-PAGE and incubated with ERCC1 polyclonal antibody (Product # PA5-77971) using a dilution of 1:1000.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Western Blot (WB)

1:500-1:3000

Product Specifications

Species Reactivity

Human, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human ERCC1. The exact sequence is proprietary.

Conjugate

Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% Proclin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2735768

Product Specific Information

Positive Control: 293T, A431, HeLa, HepG2, PC-12, Rat2

Predicted Reactivity: Mouse (86%), Pig (87%), Chimpanzee (100%), Bovine (87%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1-XPF complex makes the 5' incision.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: COFS4; complementation group 1 (includes overlapping antisense sequence); DNA excision repair protein ERCC-1; excision repair cross-complementation group 1; excision repair cross-complementing 1; excision repair cross-complementing rodent repair deficiency; excision repair cross-complementing rodent repair deficiency, complementation group 1; excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence); RAD10; UV20

Gene Aliases: COFS4; ERCC1; RAD10; UV20

UniProt ID: (Human) P07992

Entrez Gene ID: (Human) 2067, (Rat) 292673

Molecular Function: DNA binding protein endodeoxyribonuclease nuclease nucleic acid binding