Invitrogen

ERCC1 Polyclonal Antibody

Product Image Gallery

ERCC1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of ERCC1 in A431 cells. Samples were treated with 4% paraformaldehyde at RT for 15 min and incubated with ERCC1 polyclonal antibody (Product # PA5-77971) using a dilution of 1:1000, followed by Hoechst.

ERCC1 Antibody in Immunocytochemistry (ICC) — Immunofluorescence analysis of ERCC1 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ERCC1 Polyclonal Antibody (Product # PA5-77971) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

ERCC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of ERCC1 in paraffin-embedded human lung carcinoma. Sample was incubated with ERCC1 polyclonal antibody (Product # PA5-77971) using a dilution of 1:250.

ERCC1 Antibody in Western Blot (WB) — Western blot was performed using Anti-ERCC1 Polyclonal Antibody (Product # PA5-77971) and a 36 kDa band corresponding to ERCC1 was observed across the cell lines tested. An uncharacterized band of ~ 17 kDa was also observed in U-87 MG and A-431 cell lines. Modified whole cell extracts (1% SDS) (30 µg lysate) of U-87 MG (Lane1), Hep G2 (Lane 2), HeLa (Lane 3) and A-431 (Lane 4) were electrophoresed using Novex® NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Western Blot (WB)

1:500-1:3000

Product Specifications

Species Reactivity

Human, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human ERCC1. The exact sequence is proprietary.

Conjugate

Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% Proclin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2735768

Product Specific Information

Positive Control: 293T, A431, HeLa, HepG2, PC-12, Rat2

Predicted Reactivity: Mouse (86%), Pig (87%), Chimpanzee (100%), Bovine (87%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Anti-Human ERCC1 is an IgG2a Mouse Monoclonal Antibody that recognizes Excision Cross Complementing 1 (ERCC1), a human endonuclease. ERCC1 protein forms a complex with Xeroderma Pigmentosum Group F (XPF) protein. During nucleotide excision repair of damaged DNA, the ERCC1-XPF complex forms an incision at the 5' end of the DNA lesion. Deficiencies in the nucleotide excision repair pathway result in disorders such as Xeroderma pigmentosum, Cockayne's syndrome and trichothiodystrophy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DNA excision repair protein ERCC-1; excision repair cross-complementation group 1; excision repair cross-complementing 1; excision repair cross-complementing rodent repair deficiency, complementation group 1; excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)

Gene Aliases: COFS4; ERCC1; RAD10; UV20

UniProt ID: (Human) P07992

Entrez Gene ID: (Human) 2067, (Rat) 292673

Molecular Function: DNA binding protein endodeoxyribonuclease nuclease nucleic acid binding